MYOD1 Mouse Monoclonal Antibody [Clone ID: 5.8A]

CAT#: TA336376

Mouse Monoclonal MyoD Antibody (5.8A)

Product Images

Western Blot: MyoD Antibody (5.8A) TA336376 - Analysis for MyoD expression in various small round cell tumor lines using 1 ug/mL anti-MyoD mAb. The antibody only reacts with a band of approx. 45 kDa in the rhabdomyosarcoma cell line (Rh30, lane 1) but was negative against the primitive neuroectodermal (PFSK-1A, lane 2), lymphoma (EB2, lane 3), neuroblastoma (SK-N-SH, lane 4), and Ewing's sarcoma (SJSA-1, lane 5) cell lines.

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  Immunocytochemistry/Immunofluorescence: MyoD Antibody (5.8A) TA336376 - RD cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-MyoD1 (5.8A) TA336376 at a 1:200 dilution overnight at 4C and detected with and anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was counterstained with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

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  Immunohistochemistry-Frozen: MyoD Antibody (5.8A) TA336376 - Clone 5.8A antibody in human tissues. A. Rhabdomyosaroma (nuclei are stained), B. Lymphoma (staining is absent)

Specifications

Product Data

• Clone Name: 5.8A
• Applications: IHC, WB
• Recommended Dilution: Flow Cytometry, Immunohistochemistry: 1:10-1:500, Immunocytochemistry/ Immunofluorescence: 5 ug/ml, Immunoprecipitation: 1 ug/ml, Western Blot: 1 ug/ml, Immunohistochemistry-Frozen: 1:10-1:500, Knockdown Validated, Immunohistochemistry-Paraffin, Knockout Validated
• Reactivities: Human, Mouse, Rat, Feline
• Host: Mouse
• Isotype: IgG1, kappa
• Clonality: Monoclonal
• Immunogen: The 5.8A antibody was made against recombinant mouse MyoD protein but it also recognizes human (myf3), rat, and cat homologs. The epitope of this antibody was mapped to a region within aa 180-189 of mouse MyoD (NP_002469).
• Formulation: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: myogenic differentiation 1
• Database Link:
  NP_002469
  Entrez Gene 17927 MouseEntrez Gene 337868 RatEntrez Gene 4654 Human
  UniProt ID P15172
• Background: The Myogenic determination gene (MyoD) was first identified by the virtue of its ability to convert embryonic mouse fibroblast cells to muscle cells. It was subsequently shown that forced expression of MyoD (human homolog is myf 3) gene in a wide variety of normal and neoplastic cells could either convert the cells to muscle cells or activate a set of the otherwise transcriptionally inactive muscle-specific genes in these cells. The regulatory domain of the MyoD gene product lies within a 70 amino acid region and comprises a basic DNA binding motif and a helix-loop-helix (HLH) dimerization motif. Subsequent studies identified three other genes whose products shared sequence homology for the basic HLH domain of MyoD. These are; myf5, myogenin (human homolog is myf4) and myf6 (also known as MRF4 and herculin).
• Synonyms: bHLHc1; MYF3; MYOD; PUM
• Note: There is considerable literature published using the MyoD, Clone 5.8 antibody. The original development publication of the MyoD antibody, Clone 5.8A showed that the antibody detected MyoD in rhabdomysosarcomas by IHC (frozen) but not in normal adult tissues (Dias, 1992) or normal fetal skeletal muscle. The 5.8A clone also detected MyoD1 in a subset of Wilms' tumors and one ectomesenchyoma, neoplasms known to contain myogenic elements. These results led to the concept in 1992 that the 5.8A clone may be useful for differentiating rhabdomyosarcomas from other soft tissue malignancies. However, as there has been a myriad of publications since Clone 5.8A was first described, users are encourage to consult the scientific literature citing Clone 5.8A to determine the suitability of the antibody for their model system.

Reference Data

• Protein Families: Druggable Genome, Transcription Factors