IKK beta (IKBKB) Mouse Monoclonal Antibody [Clone ID: 10A9B6]

CAT#: TA336378

Mouse Monoclonal IKK beta Antibody (10A9B6)

Product Images

Flow (Intracellular): IKK beta Antibody (10A9B6) TA336378 - Analysis using the Alexa Fluor (R) 488 conjugate. Staining of IKK in HEK 293 cells using 0.1 ug of this antibody. Green histogram represents the isotype control , red represents the IKK antibody. Image using the Azide Free format of this antibody.

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  Immunoprecipitation: IKK beta Antibody (10A9B6) TA336378 - Antibody was used to immunoprecipitate IKKb from 1x10^6 Daudi cells and the immuno-precipitated protein was detected by western blotting using NB100-56509. IKKb was detected as a protein of ~87 kD. Image using the Azide Free format of this antibody.

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  Simple Western: IKK beta Antibody (10A9B6) TA336378 - Analysis using Azide Free version of TA336378. Lane view shows a specific band for IKK beta in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. *Non-specific interaction with the

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  Western Blot: IKK beta Antibody (10A9B6) TA336378 - IKK beta in Daudi cell lysate using IKK beta antibody at 5 ug/ml.

Specifications

Product Data

• Clone Name: 10A9B6
• Applications: WB
• Recommended Dilution: Immunocytochemistry/ Immunofluorescence, Gel Super Shift Assays, Immunohistochemistry-Frozen, Immunoprecipitation: 2 - 5 ug / 10^6 cells, Western Blot: 2 - 5 ug/mL, Flow Cytometry: 1:10 - 1:1000, Immunohistochemistry
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1, kappa
• Clonality: Monoclonal
• Immunogen: Full-length human IKKb/IKK2 recombinant protein was used as immunogen (NP_001547).
• Formulation: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
• Database Link:
  NP_001547
  Entrez Gene 16150 MouseEntrez Gene 3551 Human
  UniProt ID O14920
• Background: NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g. IKK1/a and IKK2/b are phosphorylated by NF-kB-inducing kinase (NIK) and MAP kinase kinase kinase-1 (MEKK1), respectively. Recent studies have shown that IKK2/b, not IKK1/a, is the target proinflammatory stimuli. Targeted disruption of IKK2/b gene in mice also results in extensive liver damage from apotopsis and death as embryo.
• Synonyms: IKK-beta; IKK2; IKKB; IMD15; NFKBIKB

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Adipocytokine signaling pathway, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Cytosolic DNA-sensing pathway, Epithelial cell signaling in Helicobacter pylori infection, Insulin signaling pathway, MAPK signaling pathway, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, Pancreatic cancer, Pathways in cancer, Prostate cancer, RIG-I-like receptor signaling pathway, Small cell lung cancer, T cell receptor signaling pathway, Toll-like receptor signaling pathway, Type II diabetes mellitus