RAD18 Mouse Monoclonal Antibody [Clone ID: 79B1048.1]

CAT#: TA336384

Mouse Monoclonal RAD18 Antibody (79B1048.1)

Product Images

Western Blot: RAD18 Antibody (79B1048.1) TA336384 - The anti-Rad18 mAb was diluted at 2 ug/ml and tested against 20 ug of HL-60 cell lysate.

Specifications

Product Data

• Clone Name: 79B1048.1
• Applications: WB
• Recommended Dilution: Western Blot: 2-4 ug/ml
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: The antibody was raised against a synthetic peptide corresponding to the amino acids 402-414 of human Rad18.
• Formulation: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: RAD18, E3 ubiquitin protein ligase
• Database Link:
  NP_064550
  Entrez Gene 56852 Human
  UniProt ID Q9NS91
• Background: Human Rad18 (hRad18) is homologue of the S. pombe Rad18 (3,4). In yeast, Rad6 and Rad18 play a crucial role in post-replication repair functions in gap-filling of a daughter strand on replication of damaged DNA (2). Furthermore, Rad6-Rad18 interaction is thought to be critical at an early step during lesion bypass in yeast. In vivo, Rad18 protein forms a tight complex with Rad6 through a conserved ring-finger motif (3,4). It has been suggested that Rad18-Rad6 complex binds to the damaged sites by the single-stranded DNA binding property of Rad 18. This is followed by degradation of the stalled components of DNA replication by ubiquitin-conjugating enzyme activity of Rad6 (1). The human Rad18 maps on chromosome 3p24-25, the region which is often deleted in lung, breast, ovary, and testis cancers. The human RAD18 gene codes for a protein of 495 amino acids with a predicted molecular weight of 56 kDa. The human Rad18 is ubiquitously expressed in various human tissues.
• Synonyms: RNF73

Reference Data

• Protein Families: Druggable Genome