TLR4 Rat Monoclonal Antibody [Clone ID: MTS510]

CAT#: TA336405

Rat Monoclonal TLR4 Antibody (MTS510)

Product Images

Flow (Cell Surface): TLR4 Antibody (MTS510) TA336405 - Analysis using Azide/BSA FREE version of NBP2-24865. Cell surface analysis of TLR4 on mouse RAW cells using 0.5 ug/10^6 cells of TLR4 antibody, anti-rat APC conjugated secondary antibody and cell surface flow kit. Green: isotype control. Red: TLR4 antibody.

• 

  Flow Cytometry: TLR4 Antibody (MTS510) TA336405 - Analysis using Azide Free version of TA336405. Cell surface TLR4 in mouse peritoneal cells. Green: isotype control. Red: anti-TLR4 antibody.

• 

  Flow Cytometry: TLR4 Antibody (MTS510) TA336405 - Analysis using the PE conjugate of NBP2-24865. Staining of cell surface TLR4 using this antibody at 0.625 ug/10^6 Raw cells (pretreated with anti-CD16/32). Shaded histogram: isotype control.

• 

  Flow Cytometry: TLR4 Antibody (MTS510) TA336405 - Analysis using the FITC conjugate of NBP2-24865. Staining of TLR4 in mouse peritoneal cells using 1 ug of this antibody. Shaded histogram: Cells without antibody; green represents isotype control; red represents anti-TLR4 antibody.

Specifications

Product Data

• Clone Name: MTS510
• Applications: FC
• Recommended Dilution: Immunocytochemistry/ Immunofluorescence, Flow Cytometry: 1-2 ug/10^6 cells, Flow (Cell Surface): 1 - 2 ug / 10^6 cells, Immunoprecipitation: 1:10-1:500
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a, kappa
• Clonality: Monoclonal
• Immunogen: This antibody was developed by immunizing rats with the Ba/F3 cell line expressing mouse TLR4 and MD-2 (Akashi et al, 2000).
• Formulation: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: toll like receptor 4
• Database Link:
  NP_612564
  Entrez Gene 21898 Mouse
  UniProt ID O00206
• Background: The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. Among this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling. The mouse TLR4 cDNA codes for a protein consisting of 835 amino acids with approximate molecular weight of 90 kDa.
• Synonyms: ARMD10; CD284; TLR-4; TOLL
• Note: Immunoprecipitation: Please see Akashi et al (2000) for details

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Pathogenic Escherichia coli infection, Toll-like receptor signaling pathway