Caspase 1 (CASP1) Rabbit Polyclonal Antibody

CAT#: TA336408

Rabbit Polyclonal Caspase-1 Antibody

Product Images

Immunohistochemistry-Paraffin: Caspase-1 Antibody TA336408 - Human reactive lymph node stained for Caspase-1 expression (TA336408) using a DAB chromogen and Hematoxylin counterstain. B is a higher magnification of A. Staining in macrophages is observed.

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  Knockout Validated: Caspase-1 Antibody TA336408 - Human epidermoid carcinoma parental cell line and Caspase-1 knockout (KO) A431 cell line. PVDF membrane was probed with 0.5 ug/ml of Mouse Anti-Human Caspase-1 Monoclonal Antibody (Catalog # TA336408) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Caspase-1 at approximately 50 kDa (as indicated) in the parental A431 cell line, but is not detectable in the knockout A431 cell line. This experiment was conducted under reducing conditions.

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  Western Blot: Caspase-1 Antibody TA336408 - Analysis of Caspase-1 using a polyclonal antibody. Human HeLa lysate probed with Caspase-1 antibody at 0.5 ug/ml.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western Blot: 0.5-2 ug/ml, Immunohistochemistry: 1:10-1:500, Immunohistochemistry-Paraffin: 1:10-1:500, Knockout Validated
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A synthetic peptide corresponding to amino acids 371-390 RKVRFSFEQPDGRAQMPTTE of human Caspase-1.
• Formulation: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: caspase 1
• Database Link:
  NP_150634
  Entrez Gene 834 Human
  UniProt ID P29466
• Background: Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis (1). The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a tetramer to form the active enzyme. Caspase-1/ICE (IL-1b converting enzyme) is similar to the cell death gene CED-3 of Caenorhabditilis elegans and regulates multiple proinflammatory cytokines, including interleukin-1b and interferon-gamma-inducing factor.
• Synonyms: ICE; IL1BC; P45
• Note: The antibody will cross-react with mouse Caspase-1, based on NCBI BLAST.

Reference Data

• Protein Families: Druggable Genome, Protease
• Protein Pathways: Amyotrophic lateral sclerosis (ALS), Cytosolic DNA-sensing pathway, NOD-like receptor signaling pathway