TLR3 Rabbit Polyclonal Antibody

CAT#: TA336412

Rabbit Polyclonal TLR3 Antibody

Product Images

Flow Cytometry: TLR3 Antibody TA336412 - Intracellular flow analysis of TLR3 in Balb/c mouse splenocytes and mouse RAW cells using TLR3 antibody (red) and isotype control antibody (green) at 1 ug/10^6 cells.

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  Flow Cytometry: TLR3 Antibody TA336412 - Analysis using the FITC conjugate of TA336412. Staining of TLR3 in Balb/c mouse splenocytes using this antibody at 1 ug/10^6 cells. Green represents rabbit IgG isotype control this antibody; red represents anti-TLR3 antibody.

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  Western Blot: TLR3 Antibody TA336412 - Analysis of TLR3 in mouse spleen tissue lysate using TA336412 at 2 ug/ml.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: Flow (Cell Surface)), Flow (Intracellular): 1 ug/1 million cells, Immunohistochemistry-Frozen: 1:20-1:1000, Immunohistochemistry-Paraffin: 1:100, Knockdown Validated, Dot Blot, Immunohistochemistry: 1:20-1:1000, Flow Cytometry: 1 ug/10^6 cells, Western Blot: 1-3 ug/ml
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A mix of synthetic peptides corresponding to amino acids 135-150 (SIHKIKSNPFKNQKNL), 828-844 (CRRFKVHHAVQQAIEQN), and 876-891 (CILNWPVQKERINAFH) of mouse TLR3.
• Formulation: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 104 kDa
• Gene Name: toll like receptor 3
• Database Link:
  NP_003256
  Entrez Gene 142980 MouseEntrez Gene 7098 Human
  UniProt ID O15455
• Background: The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules and could constitute an important and unrecognized component of innate immunity in humans. The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Ten human homologs of TLRs (TLR1-10) have been described. mTLR3 cDNA codes for a protein with approximate molecular weight of 100 kDa . TLR3 has a restricted expression pattern being expressed in dendritic cells (DC). TLR3 mRNA expression was detected by in situ hybridization in DC and lymph nodes. The expression of TLR3 in a single cell type may indicate a specific role for this molecule in a restricted setting.
• Synonyms: CD283; IIAE2
• Note: Flow Cytometry (Cell Surface): see Pawar et al. (2006) for details Flow Cytometry (Intracellular): 1 ug/10^6 cells Immunohistochemistry-Frozen: see Pwar et al, 2005 for details. Immunohistochemistry-Paraffin: 1:50 (see Patol et al, 2005 for details)

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Toll-like receptor signaling pathway