MD2 (LY96) Rabbit Polyclonal Antibody

CAT#: TA336439

Rabbit Polyclonal MD-2 Antibody

Product Images

Western Blot: MD-2 Antibody TA336439 - Image of anti-MD2. Total protein from human colon (lane 1) and human spleen (lane 2) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-MD2 in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

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  Immunohistochemistry-Paraffin: MD-2 Antibody TA336439 - Analysis of MD2 in human brain tissue using an isotype control antibody (top left) and MD2 antibody (bottom left, right) at 10 ug/ml.

Specifications

Product Data

• Applications: IHC
• Recommended Dilution: Immunohistochemistry: 1:10-1:500, Western Blot: 0.5-2 ug/ml, Immunohistochemistry-Paraffin: 5-10 ug/ml
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A full length recombinant protein was used as the immunogen.
• Formulation: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: lymphocyte antigen 96
• Database Link:
  NP_056179
  Entrez Gene 17087 MouseEntrez Gene 448830 RatEntrez Gene 23643 Human
  UniProt ID Q9Y6Y9
• Background: MD2 is a secreted glycoprotein associating with the extracellular domain of Toll-like receptor 4 (TLR4) and trafficking to and from the cell surface and the Golgi apparatus. It has a signal peptide, two N terminal glycosylation sites, and seven Cys residues and consists of a heterogeneous collection of disulfide-linked oligomers. MD2 is an essential accessory glycoprotein required for LPS recognition and associates with toll-like receptor 4 on the cell surface to confer responsiveness to lipopolysaccyaride (LPS), and hence provides a link between the receptor and LPS signaling. It can also function as a soluble receptor for cells that do not otherwise express it. MD2 contains multiple trypsin cleavage sites and its expression is correlated inversely with trypsin activity.
• Synonyms: ESOP-1; ly-96; MD-2; MD2

Reference Data

• Protein Families: Secreted Protein
• Protein Pathways: Pathogenic Escherichia coli infection, Toll-like receptor signaling pathway