Nogo A (RTN4) Rabbit Polyclonal Antibody

CAT#: TA336446

Rabbit Polyclonal Nogo Antibody

Product Images

Western Blot: Nogo Antibody TA336446 - Analysis of Nogo A and B in A) human, B) mouse, and C) rat brain tissue lysate using Nogo antibody at 1:2000.

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  Simple Western: Nogo Antibody TA336446 - Lane view shows a specific band for Nogo in 0.05 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

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  Immunohistochemistry-Paraffin: Nogo Antibody TA336446 - Analysis of human testis using Nogo antibody at 1:100.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western Blot: 1:500-1:2000, Immunohistochemistry-Paraffin: 1:100, Simple Western: 1:1000, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, ELISA
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A synthetic sequence corresponding to amino acids 14-30 (DSPPRPQPAFKYQFVRE) of human Nogo A was used as the immunogen for this antibody.
• Formulation: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Whole antisera
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: reticulon 4
• Database Link:
  NP_008939
  Entrez Gene 68585 MouseEntrez Gene 83765 RatEntrez Gene 57142 Human
  UniProt ID Q9NQC3
• Background: Nogo-B is a novel myelin-associated, intermediate-length splice variant of Nogo belonging to the reticulon family. It is a multidomain neurite growth inhibitory protein containing several discrete regions with growth inhibitory functions and is a component of the CNS myelin that prevents axonal regeneration in the adult vertebrate CNS. Nogo-B along with NGBR mediated chemotaxis in HUVECs and induced tube formation in 3-dimensional cultures. It is mostly found in intact blood vessels, smooth muscle cells, and endothelial cell, with its N terminus oriented extracellularly and is a regulator of vascular homeostasis and remodeling. It plays an important role in sporadic and familial amyotrophic lateral sclerosis
• Synonyms: 250; ASY; Nbla00271; Nbla10545; NI220; NOGO; NOGO-A; Nogo-B; Nogo-C; NOGOC; NSP; NSP-CL; RTN-X; RTN4-A
• Note: This antibody recognizes Nogo A (approximately 220 kD) and Nogo B2 and B1 (approximately 50 kD and 48 kD in human and 43 kD and 41 kD in mouse and rat, respectively).

Reference Data

• Protein Families: Transmembrane