TBK1 Mouse Monoclonal Antibody [Clone ID: 108A429]

CAT#: TA336453

Mouse Monoclonal NAK Antibody (108A429)

Product Images

Immunocytochemistry/Immunofluorescence: TBK1 Antibody (108A429) TA336453 - Antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). A dilution of 1:10 was used. Image objective 40x.

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  Immunohistochemistry-Paraffin: TBK1 Antibody (108A429) TA336453 - Immunohistochemistry: TBK1 Antibody (108A429) - Azide and BSA Free [NBP2-33243] - Staining on human testis (Formalin-Fixed Paraffin-Embedded (FFPE)) Image from the Azide and BSA Free version of this antibody.

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  Simple Western: TBK1 Antibody (108A429) TA336453 - Lane view shows a specific band for NAK/TBK1 in 0.5 mg/ml of Daudi (left) and Raw 264.7 (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody

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  Immunohistochemistry-Paraffin: TBK1 Antibody (108A429) TA336453 - Analysis of FFPE human testis tissue section using Peripheral Node Addressin antibody (clone MECA-79R) at 1:200. The staining was developed with HRP-DAB detection method and the counterstaining was performed using hematoxylin.

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  Immunocytochemistry/Immunofluorescence: TBK1 Antibody (108A429) TA336453 - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-TBK1 at 20 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

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  Western Blot: TBK1 Antibody (108A429) TA336453 - Analysis of Human Daudi (A) and mouse RAW (B) cell lysate probed with TBK1 antibody at 2 ug/ml.

• Western Blot: TBK1 Antibody (108A429) TA336453 - Analysis using Azide and BSA Free version of TA336453. Lysate from 293 cells untransfected (lane 1) and transfected with human TBK1 cDNA (lane 2) probed with TBK1 antibody at 2 ug/ml.

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Specifications

Product Data

• Clone Name: 108A429
• Applications: IHC, WB
• Recommended Dilution: Immunocytochemistry/ Immunofluorescence: 1:20, Immunohistochemistry: 1:10-1:500, Immunohistochemistry-Paraffin: 1:10-1:500, Western Blot: 1-2 ug/ml, Simple Western: 10 ug/ml, Knockout Validated, Immunoprecipitation
• Reactivities: Human, Mouse, Rat, Bovine, Canine
• Host: Mouse
• Isotype: IgG1, kappa
• Clonality: Monoclonal
• Immunogen: A synthetic peptide corresponding to amino acids 563-577 (YYQFKKDKAERRLAYC) of human TBK1 was used as immunogen for this antibody (NP_037386).
• Formulation: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: TANK binding kinase 1
• Database Link:
  NP_037386
  Entrez Gene 56480 MouseEntrez Gene 299827 RatEntrez Gene 29110 Human
  UniProt ID Q9UHD2
• Background: NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g. IKK1/a and IKK2/b are phosphorylated by NF-kB-inducing kinase (NIK) and MAP kinase kinase kinase-1 (MEKK1), respectively. Recently, a new IKK family member, TBK1 [(TANK-binding kinase), also known as NAK and T2K] has been identified. TANK (TRAF-associated NF-kB activator) interacts with TBK1 and activates NF-kB in a kinase dependent manner.
• Synonyms: NAK; T2K
• Note: In Western Blot this antibody detects a band at 80 kDa.

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Cytosolic DNA-sensing pathway, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway