TLR4 Mouse Monoclonal Antibody [Clone ID: HTA125]

CAT#: TA336463

Mouse Monoclonal TLR4 Antibody (HTA125)

Product Images

Flow Cytometry: TLR4 Antibody (HTA125) TA336463 - Analysis of TLR4 using TA336463 on PBMCs at 2ug/ 1X10^6 cells. M1 gate are unstained PBMC.

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  Flow Cytometry: TLR4 Antibody (HTA125) TA336463 - Cell surface analysis of TLR4 in stable HEK293/hTLR4 cells using TA336463 at 1 ug/10^6 cells. Secondary antibody Goat anti-Mouse IgG-PE at 0.25ug/10^6 cells.

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  Flow Cytometry: TLR4 Antibody (HTA125) TA336463 - Cell surface analysis of TLR4 in stable HEK293/hTLR4 cells using this antibody. Secondary antibody goat anti-mouse IgG-PE at 0.25ug/10^6 cells. Both stable HEK293/hTLR4 and HEK293/Vector cell lines were

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  Flow Cytometry: TLR4 Antibody (HTA125) TA336463 - Staining of human peripheral blood monocytes with Mouse anti Human CD284: Low Endotoxin.

Specifications

Product Data

• Clone Name: HTA125
• Applications: FC
• Recommended Dilution: FC: 2-3 ug/10^6 cells, IF: 1:10-1:2000, IP: 1:10-1:500
• Reactivities: Canine, Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: This antibody was developed by immunizing mice with Ba/F3 cell line expressing human TLR4 cell surface antigen.
• Formulation: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: toll like receptor 4
• Database Link:
  NP_612564
  Entrez Gene 7099 Human
  UniProt ID O00206
• Background: The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). Among this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling (4,5). The TLR4 cDNA codes for a protein consisting of 799 amino acids with approximate molecular weight of 88 kDa (6).
• Synonyms: ARMD10; CD284; TLR-4; TOLL
• Note: Confocal Microscopy: see Scheel et al. (2006) for details FA (Neutralization): please Basek et al, 2005 for details. Flow (Cell Surface): 2-5 ug/ 1x10^6 cells IF/ICC: please see Schneeman et al (2005) for details. IP: please see Shimazu et al, 1999 for details Use in vitro assays reported in scientific literature (PMID 24676500)

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Pathogenic Escherichia coli infection, Toll-like receptor signaling pathway