HIF-1 alpha (HIF1A) Rabbit Polyclonal Antibody

CAT#: TA336484

Rabbit Polyclonal HIF-1 alpha Antibody

Product Images

Simple Western: HIF-1 alpha Antibody TA336484 - Simple Western lane view shows a specific band for HIF-1 alpha in 0.5 mg/ml of Hypoxic HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

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  Immunohistochemistry-Paraffin: HIF-1 alpha Antibody TA336484 - IHC analysis of a formalin-fixed paraffin-embedded tissue section of human endometrium carcinoma AN3CA cell line based xenograft using rabbit polyclonal HIF-1 alpha antibody TA336484 at 1:300 dilution. The signal was developed using HRP-labelled secondary antibody and DAB reagent, and the section was further counterstained using hematoxylin. The tested section depicted mainly a diffused cytoplasmic staining but there were some cells which showed nuclear signal also (representing hypoxic cells).

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  Western Blot: HIF-1 alpha Antibody TA336484 - Detection of HIF-1 alpha in cobalt chloride treated/untreated COS-7 nuclear extracts using TA336484.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Knockdown Validated, Immunocytochemistry/ Immunofluorescence: 1:10 - 1:500, Immunohistochemistry-Paraffin: 1:100 - 1:300, Immunohistochemistry-Frozen: 1:100 - 1:300, Western Blot: 1:1000, Simple Western: 1:100, Immunohistochemistry: 1:100 - 1:300, Gel Super Shift Assays
• Reactivities: Human, Mouse, Rat, Porcine
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Fusion protein containing amino acids 432-528 of human HIF-1 alpha [UniProt# Q16665]
• Formulation: PBS, 0.01% Sodium Azide. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: hypoxia inducible factor 1 alpha subunit
• Database Link:
  NP_851397
  Entrez Gene 15251 MouseEntrez Gene 29560 RatEntrez Gene 3091 Human
  UniProt ID Q16665
• Background: HIF1 (hypoxia-inducible factor 1), a heterodimeric transcription factor complex central to cellular response to hypoxia, consists of two subunits (HIF-1 alpha and HIF-1 beta) which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family. Expression of HIF-1 alpha protein is regulated by cellular oxygen level alterations as well as in oxygen-independent manner via different cytokines (through the PI3K-AKT-mTOR pathway), growth factors, oncogenic activation, or loss of tumor suppressor function etc. In normoxic cells, HIF-1 alpha is proline hydroxylated leading to a conformational change that promotes its binding to the VLH (von Hippel Lindau) protein E3 ligase complex; ubiquitination and followed by rapid proteasomal degradation. Hypoxia as well as chemical hydroxylase inhibitors (desferrioxamine, cobalt etc.) inhibit HIF-1 alpha degradation and lead to its accumulation in the cells, whereas, contrastingly, HIF-1 beta/ARNT (AhR nuclear translocator) remains stable under both conditions. Besides their critical role in hypoxic response, HIF1s regulates the transcription of genes responsible for angiogenesis, erythropoiesis/iron-metabolism, glucose metabolism, cell proliferation/survival, adipogenesis, carotid body formation, B lymphocyte development and immune reactions.
• Synonyms: bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; HIF1; HIF1-ALPHA; MOP1; PASD8
• Note: This HIF-1 alpha antibody is useful for Western blot. Nuclear extracts are recommended. Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Frozen and Immunohistochemistry-Paraffin were reported in scientific literature.

Reference Data

• Protein Families: Transcription Factors
• Protein Pathways: mTOR signaling pathway, Pathways in cancer, Renal cell carcinoma