CD68 Mouse Monoclonal Antibody [Clone ID: KP1]

CAT#: TA336488

Mouse Monoclonal CD68/SR-D1 Antibody (KP1)

Product Images

Immunohistochemistry-Paraffin: CD68 Antibody (KP1) TA336488 - IHC staining of CD68 in human spleen using DAB with hematoxylin counterstain.

Specifications

Product Data

• Clone Name: KP1
• Applications: IHC
• Recommended Dilution: WB: 1:100, IHC: 1:10-1:200, IHC-F: 1:10-1:200, IHC-P: 1:10-1:200
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1, kappa
• Clonality: Monoclonal
• Immunogen: Subcellular fraction of human alveolar macrophages.
• Formulation: Preservative: 0.1% Sodium Azide. Store at 4C. Do not freeze.
• Concentration: lot specific
• Purification: Tissue culture supernatant
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: CD68 molecule
• Database Link:
  NP_001242
  Entrez Gene 12514 MouseEntrez Gene 287435 RatEntrez Gene 968 Human
  UniProt ID P34810
• Background: CD68, also known as GP110, LAMP4, and SCARD1, encodes a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissues. CD68 is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and favors binding to lectins through its heavily glycosylated extracellular domain. Furthermore, CD68 has also been found to be a member of the scavenger receptor family, whose main purpose is to clear cellular debris and regulate phagocytosis and macrophage recruitment. Recent studies have investigated the role of tumor related macrophages using CD68 as a probe (PMID: 22931216), as well as their involvement in the progression of other cancers (PMID: 21113139).
• Synonyms: GP110; LAMP4; SCARD1
• Note: This CD68 Antibody (KP1) is useful for Immunohistochemistry on frozen fixed and paraffin-emdedded sections, Immunoprecipitation, Western blot and Immunocytochemistry/Immunofluorescence. IHC: For an ABC system, dilute 1:20 and incubate for 30-60 minutes at RT. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Use in Flow Cytometry reported in scientific literature (PMID 18405323)

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Lysosome