Apolipoprotein A V (APOA5) Mouse Monoclonal Antibody [Clone ID: 4H8H8E2]

CAT#: TA336523

Mouse Monoclonal Apolipoprotein A5 Antibody (4H8H8E2)

Product Images

Immunocytochemistry/Immunofluorescence: Apolipoprotein A5 Antibody (4H8H8E2) TA336523 - Apolipoprotein A5 antibody (1:250) was tested in HepG2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Specifications

Product Data

• Clone Name: 4H8H8E2
• Recommended Dilution: Immunocytochemistry/ Immunofluorescence: 1:250, ELISA: 1:100-1:2000, Flow Cytometry
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1, kappa
• Clonality: Monoclonal
• Immunogen: Ni-NTA purified full-length recombinant human Apolipoprotein A5 expressed in E. Coli strain BL21 (DE3). [UniProt# Q6Q788]
• Formulation: PBS, pH 7.4, 0.1% Sodium Azide. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: apolipoprotein A5
• Database Link:
  NP_443200
  Entrez Gene 116519 Human
  UniProt ID Q6Q788
• Background: Apolipoprotein A-V (APOA5) belongs to apolipoprotein A1/A4/E family and is a component of several lipoprotein fractions, including VLDL, HDL and chylomicrons. Expressed at high levels in plasma and liver, APOA5 exists as a secreted protein and interacts with GPIHBP1. APOA5 is an important determinant of plasma triglyceride levels by both behaving as a potent stimulator of apo-CII lipoprotein lipase/LPL TG hydrolysis and an inhibitor of hepatic VLDL-TG production rate (without affecting the VLDL-apoB production rate). APOA5 activates lecithin: cholesterol acyltransferase (LCAT) but does not enhance efflux of cholesterol from macrophages. Transgenic mice overexpressing human APOA5 gene exhibit one-third lower plasma TG levels than wild type, whereas APOA5 knockout mice possess four times as much plasma TG levels, with no effect on plasma cholesterol levels. Clinically, defects in APOA5 are a cause of susceptibility to familial hypertriglyceridemia (FHTR) and hyperlipoproteinemia type 5 (HLPP5).
• Synonyms: APOAV; RAP3
• Note: This Apolipoprotein A5 antibody is useful in ELISA.

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: PPAR signaling pathway