TMP21 (TMED10) Rabbit Polyclonal Antibody

CAT#: TA336525

Rabbit Polyclonal Tmp21/p23 Antibody

Product Images

Immunohistochemistry-Paraffin: Tmp21/p23 Antibody TA336525 - Tmp21/p23 was detected in immersion fixed paraffin-embedded sections of human liver using Rabbit Anti-Human TMP21/p23 polyclonal Antibody (Catalog # TA336525) at 1 ug/ml for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm in hepatocytes.

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  Western Blot: TMP21 Antibody TA336525 - Detection of TMP21 in human liver lysate using TA336525.

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  Knockdown Validated: Tmp21/p23 Antibody TA336525 - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and TMP21/TMED10 knockout (KO) HEK293T cell line. PVDF membrane was probed with 2.0 ug/ml of Rabbit Anti-Human TMP21/TMED10 Polyclonal Antibody (Catalog # TA336525) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for TMP21/TMED10 at approximately 25 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reduc

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Immunohistochemistry: 1 ug/ml, Western Blot: 2 ug/ml, Immunohistochemistry-Paraffin, Knockdown Validated
• Reactivities: Human, Mouse, Rat, Bovine, Primate, Rabbit, Xenopus
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A synthetic peptide made to an internal sequence of the human TMP21 protein (within residues 50-150). [Swiss-Prot# P49755]
• Formulation: PBS, 30% glycerol, 0.1% Sodium Azide. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 21 kDa
• Gene Name: transmembrane p24 trafficking protein 10
• Database Link:
  NP_006818
  Entrez Gene 68581 MouseEntrez Gene 84599 RatEntrez Gene 10972 Human
  UniProt ID P49755
• Background: TMP21 (transmembrane protein Tmp21) is a type I transmembrane protein with large luminal domain belonging to p24 endoplasmic reticulum/Golgi cargo family and play key roles in protein transport, membrane structure organization and secretory pathway. TMP21 is ubiquitously expressed with highest protein levels observed in pancreas and intestines. In addition to its well established role in vesicle formation and cargo transport in ER/Golgi, TMP21 has been implicated in recruitment of small GTPase ADP-ribosylation factor 1 to Golgi apparatus and ADP-ribosylation factor 1-dependent assembly of actin in the Golgi. TMP21 forms a component of presenilin complex at the plasma membranes and modulates gamma -secretase cleavage. The cytoplasmic tails of p24 family interacts with coatomer (major component of coat protein complex I or COP-I), to facilitate transport between ER and Golgi. TMP21 cycles through the early secretory pathway between intermediate and cis-Golgi compartments, and is essential for proper organization of the Golgi apparatus. TMP21 also plays an essential and non-redundant role in earliest stages of mammalian development.
• Synonyms: p23; P24(DELTA); p24d1; S31I125; S31III125; Tmp-21-I; TMP21
• Note: This TMP21 antibody is useful for Immunohistochemistry and Western blot analysis, where a band is seen at ~21 kDa.

Reference Data

• Protein Families: Transmembrane