NSE (ENO2) Rabbit Polyclonal Antibody

CAT#: TA336531

Rabbit Polyclonal Enolase 2/Neuron-specific Enolase Antibody

Product Images

Western Blot: NSE Antibody TA336531 - Blot of rat spinal cord probed with rabbit antibody to NSE. The antibody stains a single sharp band corresponing to NSE at about 47kDa.

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  Western Blot: NSE Antibody TA336531 - Western blot analysis of extracts from mouse brain using NSE antibody (TA336531, 1:100).

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  Immunocytochemistry/Immunofluorescence: NSE Antibody TA336531 - Confocal immunofluorescent analysis of 3T3 cells using NSE antibody (TA336531, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green, A). Actin filame

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  Immunocytochemistry/Immunofluorescence: NSE Antibody TA336531 - Shows the human embryonic kidney cells line 293, which express many neuronal proteins (1). The red channel shows staining which recognizes all of these 293 cells. The green channels shows s

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 1:2000, IF: 1:500
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant full length human NSE purified from E. coli. [UniProt# P09104]
• Formulation: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Whole antisera
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 47 kDa
• Gene Name: enolase 2
• Database Link:
  NP_001966
  Entrez Gene 13807 MouseEntrez Gene 24334 RatEntrez Gene 2026 Human
  UniProt ID P09104
• Background: NSE (neuron-specific enolase or enolase 2) is an enzyme which facilitates the conversion of 2-phosphoglycerate to phosphoenolpyruvate in glycolytic pathway, and also the reverse reaction in gluconeogenic metabolism. It belongs to mammalian enolases ENO1, ENO2, and ENO3 (alternately known as enolase alpha, beta and gamma respectively) and these three enolases have different cell type specific expression patterns. NSE is found in relatively large amounts in neurons, PNS tissues and neuroendocrine cells, and is one of the most commonly employed biochemical markers of nervous tissue damage. Increased NSE levels in serum, CSF, or both have been reported in several neurological pathologies such as status epilepticus, Creutzfeldt-Jakob disease, acute ischemic stroke, head injury and hypoxic brain injury and Tuberculous Meningitis.
• Synonyms: HEL-S-279; NSE
• Note: This NSE antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot.

Reference Data

• Protein Pathways: Glycolysis / Gluconeogenesis, Metabolic pathways, RNA degradation