DAP5 (EIF4G2) Mouse Monoclonal Antibody [Clone ID: 39C534.1]

CAT#: TA336576

Mouse Monoclonal DAP5 Antibody (39C534.1)

Product Images

Western Blot: DAP5 Antibody (39C534.1) TA336576 - Analysis of TA336576 (1ug) on 15 ug of total cell lysate from Jurkat cells.

• 

  Flow Cytometry: DAP5 Antibody (39C534.1) TA336576 - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.

Specifications

Product Data

• Clone Name: 39C534.1
• Applications: FC, WB
• Recommended Dilution: WB: 1-2 ug/ml, FC: 1 ug per million cells
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: The antibody was raised against two synthetic peptides corresponding to amino acids 796-812 of human DAP-5 protein.
• Formulation: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: eukaryotic translation initiation factor 4 gamma 2
• Database Link:
  NP_001409
  Entrez Gene 13690 MouseEntrez Gene 1982 Human
  UniProt ID P78344
• Background: Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Recently, five novel apoptotic genes (named DAP genes for: Death Associated Proteins) have been identified that code for proteins, which display a diverse spectrum of biochemical activities. These include a novel type of calcium/calmodulin-regulated kinase, which carries ankyrin repeats and a death domain (DAP-kinase), a nucleotide-binding protein (DAP-3), a small proline-rich cytoplasmic protein (DAP-1), and a novel homolog of the eIF4G translation initiation factor (DAP-5). DAP-5 (also named p97 and NAT1) is a ubiquitously expressed member of the translation initiation factor eIF4G family that lacks the eIF4E binding site. DAP-5 was cloned from HeLa cells using a functional approach in attempt to isolate cDNA fragments, which convey resistance to gamma interferon (IFN-g)-induced programmed cell death (3). A fragment of the cDNA when expressed as a mini-protein of 28 kDa protects the cell from IFN-g induced apoptosis. The full-length DAP-5 cDNA codes for a protein of approximate molecular weight of 97 kDa. Low level of expression protects the cells, whereas high level of expression results in cell death.
• Synonyms: AAG1; DAP5; NAT1; P97

Reference Data

• Protein Families: Transcription Factors
• Protein Pathways: Viral myocarditis