p53 (TP53) Mouse Monoclonal Antibody [Clone ID: Pab 240]

CAT#: TA336582

Mouse Monoclonal p53 Antibody (PAb 240)


USD 457.00

5 Days*

Size
    • 100 ul

Frequently bought together (3)
Recombinant protein of human tumor protein p53 (TP53), transcript variant 1
    • 20 ug

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Transient overexpression lysate of tumor protein p53 (TP53), transcript variant 1
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beta Actin Mouse Monoclonal Antibody, Clone OTI1, Loading Control
    • 30 ul

USD 159.00

Specifications

Product Data
Clone Name Pab 240
Applications WB
Recommended Dilution Flow Cytometry: 1:10-1:1000, ELISA: 1:100-1:2000, Immunohistochemistry: 1:250-1:500, Immunoprecipitation: 10ug/mg, Western Blot: 1:1000-1:2000, Immunohistochemistry-Paraffin: 1:250-1:500, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence: 5 ug/ml, Immunohistochemistry-Frozen, CyTOF-ready
Reactivities Human, Mouse, Rat, Yeast (Does not react with: Xenopus)
Host Mouse
Isotype IgG1, kappa
Clonality Monoclonal
Immunogen Gel-purified p53-beta-galactosidase fusion protein containing murine p53 from aa 14-389 (derived from pSV53C cDNA clone).
Formulation PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration lot specific
Purification Protein G purified
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Gene Name tumor protein p53
Background The tumor suppressor protein p53 plays an important role in cancer development. The p53 protein is a transcription factor that acts as a checkpoint in the cell cycle, either preventing or initiating programmed cell death. Since cancer occurs as the malfunction in the proliferation of cells, disrupting p53 activity allows abnormal cells to multiply or allows a tumor's progression from benign to malignant. This antibody detects p53 in all mammals tested. Detection of p53 using this antibody: 50% of colon cancer sections are positive, 30% of breast cancer sections and 70% of lung cancer sections. Normal and premalignant tissues are negative.
Synonyms BCC7; LFS1; P53; TRP53
Note Antigen retrieval with IHC-P is not essential but may optimise staining. In IP this antibody reacts with only mutant p53 protein under non-denaturing conditions. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 1394225) Use in Immunohistochemistry-Frozen reported in various pieces of scientific literature.
Reference Data
Protein Families Druggable Genome, Stem cell - Pluripotency, Transcription Factors
Protein Pathways Amyotrophic lateral sclerosis (ALS), Apoptosis, Basal cell carcinoma, Bladder cancer, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, Glioma, Huntington's disease, MAPK signaling pathway, Melanoma, Neurotrophin signaling pathway, Non-small cell lung cancer, p53 signaling pathway, Pancreatic cancer, Pathways in cancer, Prostate cancer, Small cell lung cancer, Thyroid cancer, Wnt signaling pathway

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.