Neurofilament (NEFL) Rabbit Polyclonal Antibody

CAT#: TA336601

Rabbit Polyclonal NF-L Antibody

Product Images

Western Blot: 68kDa Neurofilament Light Antibody TA336601 - Western blot of whole rat cerebellum homogenate stained with TA336601, at dilution of 1:20,000. A prominent band running with an apparent SDS-PAGE molecular weight of ~68kDa corresponds to rode

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  Immunocytochemistry/Immunofluorescence: 68kDa Neurofilament Light Antibody TA336601 - Mixed neuron/glia cultures from newborn rat brain stained with NB300-220 antibody to peripherin (green) and rabbit polyclonal antibody to NF-L RPCA-NF-L (red channel).

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 1:20000, IF: 1:500-1:5000, IHC: 1:5000, IHC-P: 1:5000
• Reactivities: Human, Mouse, Rat, Avian, Bovine, Mammal, Porcine
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Native Neurofilament Light protein purified from pig spinal cord [UniProt# P02547]
• Formulation: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Whole antisera
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 68 kDa
• Gene Name: neurofilament, light polypeptide
• Database Link:
  NP_006149
  Entrez Gene 18039 MouseEntrez Gene 83613 RatEntrez Gene 4747 Human
  UniProt ID P07196
• Background: Neuronal intermediate filaments or neurofilaments are found specifically in neurons where they play a key role in maintaining neuronal morphology as well as in regenerating myelinated axons. Neurofilaments contain three subunits: a light polypeptide (NEFL/NFL), a medium polypeptide (NEFM/NFM), and a heavy polypeptide (NEFH/NFH), with molecular weights of 68, 160, and 212 kD respectively. 68kD Neurofilament Light (NEFL) is the most abundant of three neurofilament proteins and, like other nonepithelial intermediate filament proteins, it can form homopolymeric 10-nm filaments. NEFL forms the backbone of neurofilament and perturbations in its expression leads to motor neuron diseases, such as Charcot-Marie-Tooth disease, type 2E (CMT2E). In addition to its influence on the nervous system, NEFL has been shown to act as a tumor suppressor and has been linked to carcinogenesis/metastasis of several malignancues including breast, prostate, lung, colon, and bladder cancer.
• Synonyms: CMT1F; CMT2E; NF-L; NF68; NFL; PPP1R110
• Note: This 68kDa Neurofilament Light antibody is useful in Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections and Western blot.

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS
• Protein Pathways: Amyotrophic lateral sclerosis (ALS)