NFH (NEFH) Mouse Monoclonal Antibody [Clone ID: NAP4]

CAT#: TA336606

Mouse Monoclonal 200kDa Neurofilament Heavy Antibody (NAP4)

Product Images

Western Blot: 200kDa Neurofilament Heavy Antibody (NAP4) TA336606 - Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stainted with various antibod

Specifications

Product Data

• Clone Name: NAP4
• Applications: WB
• Recommended Dilution: WB: 1:5000, ELISA: 1:100-1:2000, IHC: 1:1000, IHC-P: 1:1000
• Reactivities: Human, Mouse, Rat, Avian, Mammal
• Host: Mouse
• Isotype: IgG1, kappa
• Clonality: Monoclonal
• Immunogen: Native Neurofilament Heavy purified from pig spinal cord [UniProt# P12037]
• Formulation: Preservative: 0.05% Sodium Azide. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Ascites
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 210 kDa
• Gene Name: neurofilament, heavy polypeptide
• Database Link:
  NP_066554
  Entrez Gene 24587 RatEntrez Gene 380684 MouseEntrez Gene 4744 Human
  UniProt ID P12036
• Background: Neuronal intermediate filaments (NIF) are found specifically on neurons where they play key role in maintaining neuronal morphology as well as in regenerating myelinated axons. NIF contain three subunits: a light polypeptide (NEFL/NFL), a medium polypeptide (NEFM/NFM), and a heavy polypeptide (NEFH/NFH), with molecular weights of 68, 160, and 212 kD respectively. 200kD neurofilament heavy (NEFH) is one of the major components of neuronal cytoskeleton NIF and NEFH gene was originally proposed as a DNA marker for presymptomatic diagnosis in neurofibromatosis type 2 (NF2). NEHF facilitate interactions between NIF and brain mitochondria with binding efficiency dependent upon mitochondrial membranes potential. NEFH and beta-catenin are constituents in postsynaptic density (PSD) in CNS and NEFH is essential for maintaining normal cell integrity. Diminished NEFH levels are seen in certain cancers including human autonomic nerve tumors or central neurocytomas, prostate cancer, clear-cell epithelioid tumor and small cell lung carcinoma. Fibroblasts overexpressing NEFH are distorted, multinucleated, and possess inclusions, typical changes seen in neuropathies, and defects in NEFH are linked to susceptibility to ALS.
• Synonyms: NFH
• Note: This 200kDa Neurofilament Heavy antibody can be used for ELISA, Immunohistochemistry on paraffin-embedded sections and Western blot.

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Amyotrophic lateral sclerosis (ALS)