Peripherin (PRPH) Rabbit Polyclonal Antibody

CAT#: TA336607

Rabbit Polyclonal Peripherin Antibody

Product Images

Western Blot: Peripherin Antibody TA336607 Detection of the 57 kDa peripherin protein in whole rat brain stem homogenate using NB 300-137 (1:20,000). The faint 48 kDa band is a peripherin molecule.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:5000, ELISA: 1:100-1:2000, IF: 1:1000, IHC: 1:500, IHC-F: , IHC-P:
• Reactivities: Human, Mouse, Rat, Mammal
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant full length rat Peripherin [UniProt# P21807]
• Formulation: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Whole antisera
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 57 kDa
• Gene Name: peripherin
• Database Link:
  NP_006253
  Entrez Gene 19132 MouseEntrez Gene 24688 RatEntrez Gene 5630 Human
  UniProt ID P41219
• Background: Peripherin (PRPH) is a 57kD type III neuronal intermediate filament (NIF) protein which is predominantly expressed in neurons of PNS and in certain CNS neurons specifically from brain stem/spinal cord which generally project toward peripheral structures. Inside developing neurons, PRPH is expressed abundantly in the growth cones and is required for axonal outgrowth as well as maintenance or regeneration. In mature neurons, together with other NIFs, PRPH constitutes a shape-maintaining IF network. Besides its crucial role in development of nervous system, PRPH can also be highly relevant to pathogenesis of neurodegenerative disorder. PRPH expression is induced in CNS neurons (wherein it is not expressed normally) upon brain injury, and factors such as LIF, IL-6 etc and PRPH molecules form aggregates in neurons. Synaptic plasticity is markedly altered in PRPH transgenic mice (Per mice) and up-regulation of PRPH expression is implicated in some pathophysiological conditions, such as cerebral ischemia and seizure, and some cognitive disorders. In ALS patients, PRPH together with neuronal IFs are detected in the majority of abnormal IF inclusion bodies in mature or aging motor neurons.
• Synonyms: NEF4; PRPH1
• Note: This Peripherin antibody can be used for ELISA, Immunocytochemistry/Immunofluorescence and Western blotting. In WB a band can be seen at ~57 kDa. Success in IHC on mouse tissue has been reported in a customer review. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 16325747) Use in Immunohistochemistry-frozen reported in scientific literature (PMID 24277578)

Reference Data

• Protein Pathways: Amyotrophic lateral sclerosis (ALS)