GAPDH Rabbit Polyclonal Antibody

CAT#: TA336634

Rabbit Polyclonal GAPDH/G3PDH Antibody


USD 410.00

5 Days*

Size
    • 100 ul

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Specifications

Product Data
Applications WB
Recommended Dilution WB: 1:2000-1:10000
Reactivities Mouse
Host Rabbit
Clonality Polyclonal
Immunogen The epitope recognized by this antibody maps to a region between residues 300 and the C-terminus (residue 335) of mouse GAPDH using the numbering given in entry NP_001001303.1 (GeneID 407972).
Formulation Tris-citrate/phosphate, pH 7, 0.1% Sodium azide. Store at 4C. Do not freeze.
Concentration lot specific
Purification Immunogen affinity purified
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Predicted Protein Size 38 kDa
Gene Name glyceraldehyde-3-phosphate dehydrogenase
Background GAPDH (glyceraldehyde-3-phosphate dehydrogenase or GAPD) is a key enzyme in glycolytic pathway, wherein it catalyzes the first step by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. It localizes mainly in the cytoplasm from where it translocates to nucleus following S-nitrosylation and interaction with SIAH1. Nuclear GAPDH implicates in transcription, RNA transport, DNA replication and apoptosis, via its nitrosylase activity which mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2, PRKDC etc. GAPDH also regulates the organization/assembly of cytoskeleton and facilitates CHP1-dependent microtubule - membrane associations. It is a component of GAIT (gamma interferon-activated inhibitor of translation) complex which mediates IFN-gamma-induced transcript-selective translation inhibition in inflammation. Because of its expression as housekeeping protein in most cell types, GAPDH is often used as a control molecule in various genes expression studies, however, recent evidence has shown the association of its altered expression with neurodegenerative pathologies such as Huntington disease, Alzheimer's disease etc., and elevated GAPDH mRNA/protein expression levels have been seen in pancreatic, lung and prostate cancers.
Synonyms G3PD; GAPD; HEL-S-162eP
Note This GAPDH antibody is useful for Western blot, where a band is observed ~36 kDa. The antibody does not work in Immunoprecipitation.
Reference Data
Protein Families ES Cell Differentiation/IPS
Protein Pathways Alzheimer's disease, Glycolysis / Gluconeogenesis, Metabolic pathways

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