GAPDH Mouse Monoclonal Antibody [Clone ID: 2D4A7]

CAT#: TA336636

Mouse Monoclonal GAPDH/G3PDH Antibody (2D4A7)

Product Images

Western Blot: GAPDH Antibody (2D4A7) TA336636 - Analysis using anti-GAPDH monoclonal antibody, against cell lysate (2: Hela, 3: K562, 4: Jurkat, 5: NIH/3T3, 6: HepG-2).

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  Western Blot: GAPDH Antibody (2D4A7) TA336636 - Detection of GAPDH in mouse liver.

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  Western Blot: GAPDH Antibody (2D4A7) TA336636 - Western blot analysis of HeLa cell lysate using GAPDH antibody (TA336636) at 1:1000.

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  Immunocytochemistry/Immunofluorescence: GAPDH Antibody (2D4A7) TA336636 - Confocal immunofluorescence analysis of HeLa cells using GAPDH antibody at 1:50 (green). Nuclei were counterstained using DAPI (blue).

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  Immunocytochemistry/Immunofluorescence: GAPDH Antibody (2D4A7) TA336636 - Detection of GAPDH (Green) in Hela cells using TA336636 at a 1:200 dilution. Nuclei (Blue) were counterstained using Hoechst 33258.

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  Immunocytochemistry/Immunofluorescence: GAPDH Antibody (2D4A7) TA336636 - IF Confocal analysis of Hela cells using GAPDH antibody (TA336636, 1:50). An Alexa Fluor 488-conjugated Goat to mouse IgG was used as secondary antibody (green, A). Actin filament

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Specifications

Product Data

• Clone Name: 2D4A7
• Applications: IF, WB
• Recommended Dilution: WB: 1:20000, ELISA: 1:100-1:2000, IF: 1:200-1:1000, IHC: 1:1000-1:4000, IHC-P: 1:1000-1:4000
• Reactivities: Human, Mouse, Porcine, Rabbit, Rat
• Host: Mouse
• Isotype: IgG1, kappa
• Clonality: Monoclonal
• Immunogen: Ni-NTA purified recombinant human GAPDH expressed in E. Coli strain BL21 (DE3). [UniProt# P04406]
• Formulation: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 38 kDa
• Gene Name: glyceraldehyde-3-phosphate dehydrogenase
• Database Link:
  NP_002037
  Entrez Gene 14433 MouseEntrez Gene 24383 RatEntrez Gene 2597 Human
  UniProt ID P04406
• Background: GAPDH (glyceraldehyde-3-phosphate dehydrogenase or GAPD) is a key enzyme in glycolytic pathway, wherein it catalyzes the first step by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. It localizes mainly in the cytoplasm from where it translocates to nucleus following S-nitrosylation and interaction with SIAH1. Nuclear GAPDH implicates in transcription, RNA transport, DNA replication and apoptosis, via its nitrosylase activity which mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2, PRKDC etc. GAPDH also regulates the organization/assembly of cytoskeleton and facilitates CHP1-dependent microtubule - membrane associations. It is a component of GAIT (gamma interferon-activated inhibitor of translation) complex which mediates IFN-gamma-induced transcript-selective translation inhibition in inflammation. Because of its expression as housekeeping protein in most cell types, GAPDH is often used as a control molecule in various genes expression studies, however, recent evidence has shown the association of its altered expression with neurodegenerative pathologies such as Huntington disease, Alzheimer's disease etc., and elevated GAPDH mRNA/protein expression levels have been seen in pancreatic, lung and prostate cancers.
• Synonyms: G3PD; GAPD; HEL-S-162eP
• Note: This GAPDH antibody is useful for ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections and Western Blot.

Reference Data

• Protein Families: ES Cell Differentiation/IPS
• Protein Pathways: Alzheimer's disease, Glycolysis / Gluconeogenesis, Metabolic pathways