GRP78 (HSPA5) Rabbit Polyclonal Antibody

CAT#: TA336638

Rabbit Polyclonal GRP78/HSPA5 Antibody


USD 424.00

5 Days*

Size
    • 100 ul

Product Images

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Specifications

Product Data
Applications WB
Recommended Dilution Immunocytochemistry/ Immunofluorescence: 1:20, Simple Western: 1:25, Western Blot: 2 ug/ml, Immunohistochemistry-Paraffin, Immunohistochemistry
Reactivities Mouse, Rat (Does not react with: Human, Primate)
Host Rabbit
Clonality Polyclonal
Immunogen A synthetic peptide made to a C-terminal portion of rat GRP78 (within residues 600-654). [UniProt# P06761]
Formulation PBS, 30% glycerol, 0.1% Sodium Azide. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Concentration lot specific
Purification Immunogen affinity purified
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Predicted Protein Size 78 kDa
Gene Name heat shock protein family A (Hsp70) member 5
Background Within the ER misfolded proteins are detected by Bip(GPR78). In the presence of misfolded proteins, Bip disscociates from PERK, allowing it to homodimerize and autophosphoyate. In its dimerized, phosphorylated form PERK phosphorylates eIF2. The phosphorylation of eIF2 blocks the majority of translation to prevent the continued accumulation of protein in the ER. Bip also is binds to IRE1 and ATF6 under normal ER conditions, but dissociates upon accumulation of misfolded proteins. Unbound ATF6 is translocated to the gogli where it is converted into a cleaved, active form. The cleaved form of ATF6 is then able to up regulated transcription of UPR genes including XBP1. Activated IRE1 acts as an endoribonuclease to an intron from XBP1 transcripts. The XBP1 splice variant codes for an active transcription factor which activates transcription of P58IPK. P58IPK is a HSP40 protein which binds to and inhibits PERK. Thus, if the accumulated, misfolded proteins have been removed, P58IPK acts to shut down the UPR by removing the block to translation.
Synonyms BIP; GRP78; HEL-S-89n; MIF2
Note This GRP78 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot. In Western blot this antibody detects a 78 kDa protein representing GRP 78 from rat liver extract and HeLa cells. In ICC/IF, cytoplasmic staining was observed in HeLa cells.
Reference Data
Protein Families Druggable Genome
Protein Pathways Antigen processing and presentation, Prion diseases

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.