Fatty Acid Synthase (FASN) Rabbit Polyclonal Antibody
Frequently bought together (3)
Recombinant protein of human fatty acid synthase (FASN)
USD 867.00
Other products for "Fatty Acid Synthase"
Specifications
Product Data | |
Applications | IHC, WB |
Recommended Dilution | Knockdown Validated, Immunohistochemistry: 1:500, Immunocytochemistry/ Immunofluorescence: 1:2000, Immunoprecipitation: 1:100, Western Blot: 1:1000, Immunohistochemistry-Paraffin: 1:500immunoprecipitation 1:100 |
Reactivities | Human, Mouse, Rat, Chicken, Hamster, Porcine, Primate |
Host | Rabbit |
Clonality | Polyclonal |
Immunogen | A synthetic peptide, conjugated to KLH, made near the N-terminus of mouse FAS. [Swiss-Prot# P19096] |
Formulation | Tris-citrate/phosphate, pH 7, 0.1% Sodium azide. Store at 4C. Do not freeze. |
Concentration | lot specific |
Purification | Whole antisera |
Conjugation | Unconjugated |
Storage | Store at -20°C as received. |
Stability | Stable for 12 months from date of receipt. |
Predicted Protein Size | 272 kDa |
Gene Name | fatty acid synthase |
Database Link | |
Background | Fatty acid synthetase (FASN) is a key player of fatty acid biosynthetic pathway and it catalyzes formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH (de novo lipid synthesis). Localized in cytoplasm and melanosomes, FASN is expressed at low level in normal adult tissues; however, it is significantly upregulated in cancer cells. The FASN expression is regulated by growth factors/growth factor receptors (EGFR and HER2) and steroid hormone /steroid hormone receptors (ER, AR and progesterone receptor). Downstream of the receptors, PI3K-AKT and MAPK signaling mediate FASN expression through SREBP-1c. FASN expression is also controlled by p53 family proteins and SPOT14, lipogenesis-related nuclear protein generally overexpressed in breast cancer. Extracellular stresses including hypoxia and low pH also induce the FASN expression in cancer cells and USP2a stabilises FASN alongwith promotion of its mTOR-dependent selective translational induction. FASN activation provides rapidly proliferating tumor cells sufficient amount of lipids for membrane biogenesis and confers growth/survival advantage possibly acting as a metabolic oncogene. Because FASN-inhibitors are selectively toxic to tumor cells, it has emerged as an attractive target for cancer. |
Synonyms | FAS; OA-519; SDR27X1 |
Note | This Fatty Acid Synthase antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation and Western Blot where a band at ~272 kDa is observed. May see 1 or 2 minor cross-reacting lower MW bands in liver tissue. In ICC/IF cytoplasmic staining can be seen in MCF7 cells. |
Reference Data | |
Protein Families | Druggable Genome |
Protein Pathways | Fatty acid biosynthesis, Insulin signaling pathway, Metabolic pathways |
Documents
Product Manuals |
FAQs |
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