LIMPII (SCARB2) Rabbit Polyclonal Antibody

CAT#: TA336643

Rabbit Polyclonal LIMPII/lpg85 Antibody

Product Images

Immunocytochemistry/Immunofluorescence: LIMPII/SR-B2 Antibody TA336643 - Detection of LIMPII/lpg85 (Green) in Hela cells using TA336643. Nuclei (Blue) are counterstained using Hoechst 33258.

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  Western Blot: LIMPII/SR-B2 Antibody TA336643 - Detection of LIMP II (~85 kDa) in Lane 1: Rat cardiac fibroblasts (RCF), 3T3 mouse fibroblasts (2), canine lung lysate (3), RCF treated with angiotensin II (4), and H2O2 (5), HEPG2 human liver lysate (6).

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  Immunohistochemistry: LIMPII/SR-B2 Antibody TA336643 - Staining of LIMP II in rat infarctzone tissue with a DAB detection system.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Immunohistochemistry: 1:200, Immunocytochemistry/ Immunofluorescence: 1:50-1:250, Immunoprecipitation: 1:100, Western Blot: 1:1000, Simple Western, Immunohistochemistry-Paraffin
• Reactivities: Human, Mouse, Rat, Canine, Primate
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A C-terminal synthetic peptide made to the mouse LIMPII/lgp85 protein sequence. [UniProt# O35114]
• Formulation: Tris-citrate/phosphate, pH 7, 0.1% Sodium azide. Store at 4C. Do not freeze.
• Concentration: lot specific
• Purification: Whole antisera
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 85 kDa
• Gene Name: scavenger receptor class B member 2
• Database Link:
  NP_005497
  Entrez Gene 12492 MouseEntrez Gene 117106 RatEntrez Gene 950 Human
  UniProt ID Q14108
• Background: LIMP II (lysosome membrane protein 2; also called LGP85, SCARB2, CD36 antigen-like 2/CD36L2) is a member of the scavenger receptor class B member 2 family, ubiquitously expressed in higher organisms as a major lysosomal and endosomal membrane protein. LIMP II localize primarily to late endosomes/lysosomes as type III transmembrane protein, which traverses the membrane twice with the uncleaved signal peptide and a hydrophobic region near to the COOH-terminus, followed by twenty-amino acid long cytoplasmic tail of which a large portion located in lysosomal lumenal side possess 10-11 N-glycosylation sites. LIMP II acts as a lysosomal receptor for glucosylceramidase (GBA) targeting and it plays a key role in regulating the biogenesis of the endosomal/lysosomal compartments (ELCs). LIMP II serves as one of the major components of the molecular machinery required for intercompartmental membrane trafficking along the endocytic pathway rather than solely serving as a structural constituent of late endosomes and lysosomes. LIMP II deficiency has been found to result in the development of an obstruction of ureteric pelvic junction, deafness and peripheral neuropathy.
• Synonyms: AMRF; CD36L2; EPM4; HLGP85; LGP85; LIMP-2; LIMPII; SR-BII
• Note: This LIMPII/lpg85 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunoprecipitation and Western Blot, where a band is observed at ~85 kDa. Use in IHC-P was reported in scientific literature (PMID: 23863627).

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Lysosome