IL23 Receptor (IL23R) Rabbit Polyclonal Antibody

CAT#: TA336672

Rabbit Polyclonal IL-23 R Antibody

Product Images

Immunohistochemistry-Paraffin: IL-23 R Antibody [NB600-1147] - Analysis of Mouse spleen tissue probed with isotype control antibody (top) and IL-23R antibody (bottom) at 5 ug/ml.

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  Simple Western: IL-23 R Antibody [NB600-1147] - Lane view shows a specific band for IL23 Receptor in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

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  Immunohistochemistry-Paraffin: IL-23 R Antibody [NB600-1147] - Staining of IL-23 Receptor in Human periarterial lymphatic sheath lymphocytes (spleen) at 10 ug/ml. Hematoxylin-eosin counterstain.

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  Western Blot: IL-23 R Antibody [NB600-1147] - Analysis of IL-23R in (A) Jurkat and (B) NIH 3T3 cells. 1:500 dilution.

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  Western Blot: IL-23 R Antibody [NB600-1147] - Analysis using IL-23R antibody. Lysate from (A) human Jurkat and (B) Mouse NIH 3T3 cells probed with IL-23R antibody at 0.5 ug/ml.

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Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Flow (Cell Surface), Immunohistochemistry-Paraffin: 5-10 ug/ml, Simple Western: 1:10, Flow Cytometry: 1:10-1:1000, Immunohistochemistry: 5-10 ug/ml, Western Blot: 0.5-1.0 ug/ml
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide located at amino acids 439-455 (EIFIPEHKPTDYKKENT) of human IL-23R (NP_653302).
• Formulation: PBS with 0.09% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 72 kDa
• Gene Name: interleukin 23 receptor
• Database Link:
  NP_653302
  Entrez Gene 209590 MouseEntrez Gene 149233 Human
  UniProt ID Q5VWK5
• Background: IL-23 is a heterodimeric cytokine that shares biological properties with proinflammatory cytokines. IL-23 is composed of the IL-12p40 soluble receptor subunit and a novel cytokine-like subunit related to IL-12p35, termed p19. IL-23 mediates it cellular function by binding to IL-23R, which pairs with IL-12Rb1 to confer IL-23 responsiveness. However, human IL-12 does not bind to human IL-23R. IL-23 has been shown to possess biological activities on T cells that are similar as well distinct from those of IL-12.
• Synonyms: interleukin 23 receptor; OTTHUMP00000010660
• Note: Based on immunogen sequence, this antibody will recognize the 629 amino acid (~68 KDa) , 390 AA (~42 KDa) and 227 AA (~26 KDa) forms of human IL-23R. Flow (Cell Surface): See Kortylweski et al, 2009 for details. Use in Flow cell surface reported in scientific literature (PMID 24358364)

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway