beta Actin (ACTB) Rabbit Polyclonal Antibody

CAT#: TA336689

Rabbit Polyclonal beta-Actin Antibody


USD 479.00

5 Days*

Size
    • 50 ul

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Specifications

Product Data
Applications IHC, WB
Recommended Dilution Immunohistochemistry-Paraffin: 1:100, Proximity Ligation Assay, Simple Western: 1:12.5, Immunohistochemistry: 1:100, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation: 1:10-1:500, Western Blot: 1:100-1:2000, Flow Cytometry: 1:10-1:1000, Block/Neutralize, ELISA
Reactivities Human, Mouse, Rat, Porcine, Avian, Hamster
Host Rabbit
Clonality Polyclonal
Immunogen A synthetic peptide made to an N-terminal region of human Beta Actin. [UniProt P60709].
Formulation Tris-citrate/phosphate, pH 7, 0.1% Sodium azide. Store at 4C. Do not freeze.
Concentration lot specific
Purification Immunogen affinity purified
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Gene Name actin, beta
Background Beta actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta actin and the static and dynamic aspects of the cytoskeleton.
Synonyms BRWS1; PS1TP5BP1
Note Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Immunocytochemistry/Immunofluorescence, Blocking/Neutralizing and Enzyme Immunoassay were reported in scientific literature. Customer review using ICC. Use in Proximity Ligation Assay reported in scientific literature (PMID 24740405)
Reference Data
Protein Families ES Cell Differentiation/IPS
Protein Pathways Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy, Focal adhesion, Hypertrophic cardiomyopathy (HCM), Leukocyte transendothelial migration, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton, Tight junction, Vibrio cholerae infection, Viral myocarditis

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.