SMAD1 Rabbit Polyclonal Antibody

CAT#: TA336694

Rabbit Polyclonal SMAD1/5/8/9 Antibody

Product Images

Western Blot: SMAD1/5/8/9 Antibody TA336694 - Analysis using SMAD antibody. Lysate from A549 cells probed with antibody at 0.5 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western Blot: 0.5 - 2 ug/ml
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: This antibody was raised against two synthetic peptides corresponding to amino acids 18-32 (LGWKQGDEEEKWAEK) and amino acids 315-329 (SNVNRNSTIENTRRH) of human SMAD1. GenBank Accession No. AAP36050.1. They are 100% conserved or highly conserved between
• Formulation: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: SMAD family member 1
• Database Link:
  NP_005891
  Entrez Gene 17125 MouseEntrez Gene 25671 RatEntrez Gene 4086 Human
  UniProt ID Q15797
• Background: SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily (1). SMADs can be divided into receptor-regulated SMADs (R-SMADs: SMAD1, 2, 5, 8 and 9), common-mediator SMAD (co-SMAD: SMAD4), and inhibitory SMADs (I-SMADs: SMAD6 and 7). SMAD1, 5, 8 and 9 have high degrees of homology and antibodies are available that recognize sequences common to all of them. SMAD8 and SMAD9 are typically used as alternate names for one another in the literature.
• Synonyms: BSP-1; BSP1; JV4-1; JV41; MADH1; MADR1
• Note: Western Blot: Detects a band of approximately 52 kDa.

Reference Data

• Protein Families: Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Stem cell relevant signaling - JAK/STAT signaling pathway, Stem cell relevant signaling - TGFb/BMP signaling pathway, Transcription Factors
• Protein Pathways: TGF-beta signaling pathway