Beclin 1 (BECN1) Mouse Monoclonal Antibody [Clone ID: 9B6]

CAT#: TA336698

Mouse Monoclonal Beclin 1/ATG6 Antibody (9B6)

Product Images

Western Blot: Beclin 1 Antibody (9B6) TA336698 - HeLa whole cell extract.

Specifications

Product Data

• Clone Name: 9B6
• Applications: WB
• Recommended Dilution: WB: 1:1000, IHC: 1:50- 1:100
• Reactivities: Human, Mouse, Rat, Bovine, Chicken, Equine, Porcin
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: Full length human Beclin 1 protein [Swiss-Prot# Q14457]
• Formulation: Preservative: 0.05% Sodium Azide. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Ascites
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 52 kDa
• Gene Name: beclin 1
• Database Link:
  NP_003757
  Entrez Gene 56208 MouseEntrez Gene 114558 RatEntrez Gene 8678 Human
  UniProt ID Q14457
• Background: Beclin 1 is the first identified mammalian gene to mediate autophagy and also has tumor suppressor and antiviral function. Autophagy, a process of bulk protein degradation through an autophagosomic-lysosomal pathway, is important for differentiation, survival during nutrient deprivation, and normal growth control, and is often defective in tumor cells. Beclin 1 was originally isolated in a yeast two hybrid screen to identify Bcl-2-binding partners and maps to a tumor susceptibility locus on human chromosome 17q21 that is frequently monoallelically deleted in human breast, ovarian and prostate cancer. Beclin 1 encodes an evolutionarily conserved 60 kDa coiled coil protein that is expressed in human muscle, epithelial cells and neurons. In gene transfer studies, beclin 1 promotes nutrient deprivation-induced autophagy, inhibits mammary tumorigenesis, and inhibits viral replication. Expression of the Beclin 1 protein is frequently decreased in malignant breast epithelial cells. Based upon these observations, it is speculated that beclin 1 may work through induction of autophagy to negatively regulate tumorigenesis and to control viral infections. Beclin 1 may also play a role in other biological processes in which autophagy is important such as cell differentiation and nutritional stress responses.
• Synonyms: ATG6; beclin1; VPS30
• Note: This Beclin 1 antibody is useful for Immunohistochemistry and Western blot, where a band is seen ~52 kDa.

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Regulation of autophagy