Macrophage Scavenger Receptor I (MSR1) Rabbit Polyclonal Antibody

CAT#: TA336699

Rabbit Polyclonal SR-AI/MSR Antibody

Product Images

Immunocytochemistry/Immunofluorescence: SR-AI/MSR Antibody TA336699 - The MSR antibody was tested in Raw cells at a 1:250 dilution against DyLight 488 (Green). Alpha-tubulin and nuclei were counterstained against DyLight 550 (Red) and DAPI (Blue), respectively.

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  Flow Cytometry: SR-AI/MSR Antibody TA336699 - A surface stain was performed on Raw264.7 cells with SR-AI/MSR Antibody TA336699R (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to DyLight 550.

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  Western Blot: SR-AI/MSR Antibody TA336699 - Detection of Macrophage Scavenger Receptor I (MSR1) protein in the lysate of human liver.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Proximity Ligation Assay, Immunohistochemistry, Western Blot: 0.5 ug/ml, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence: 1:50-1:200, Immunohistochemistry-Frozen, Flow Cytometry: 2-5 ug/million cells
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide made to an internal portion of human Macrophage Scavenger Receptor I (within residues 400-450). [Swiss-Prot# P21757]
• Formulation: PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 50 kDa
• Gene Name: macrophage scavenger receptor 1
• Database Link:
  NP_619729
  Entrez Gene 20288 MouseEntrez Gene 4481 Human
  UniProt ID P21757
• Background: Macrophage Scavenger Receptor I (MSR1, also called CD204 or scavenger receptor A) belongs to scavenger receptor A family and represents phagocytic pattern-recognition receptors (PRRs) expressed predominantly on macrophages and dendritic cells. MSR1 is a single-pass type II membrane glycoprotein capable of binding to a wide range of polyanionic ligands (e.g., modified low density lipoproteins, pathogen-derived molecules), and is implicated in the pathologic deposition of cholesterol in arterial walls during atherogenesis, innate immune response as well as Alzheimer's disease. MSR1 mediates the endocytosis of a diverse group of macromolecules including modified LDLs, and scavenger receptors MSR1, CD36 and LOX-1 are three main receptors for oxidized LDL on macrophage membrane wherein MSR1/CD36 account for more than 9/10 of the lipid accumulation in macrophages exposed to oxidized LDL. Defects in MSR1 have also been linked to prostate cancer and Barrett esophagus (BE).
• Synonyms: CD204; phSR1; phSR2; SCARA1; SR-A; SRA
• Note: This Macrophage Scavenger Receptor I antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen ~50 kDa.

Reference Data

• Protein Families: Druggable Genome, Transmembrane