alpha Synuclein (SNCA) Mouse Monoclonal Antibody [Clone ID: 2A7]

CAT#: TA336706

Mouse Monoclonal Synuclein-alpha Antibody (2A7)

Product Images

Western Blot: alpha-Synuclein Antibody (2A7) TA336706 - Blots of TA336706 on recombinant alpha-synuclein (left lane) and crude extract of mouse brain (right lane, courtesy Rogan Tinsley, Howard Florey Institute, University of Melbourne).

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  Immunocytochemistry/Immunofluorescence: alpha-Synuclein Antibody (2A7) TA336706 - Mixed neuron-glial cultures stained with TA336706, our monoclonal antibody to alpha-synuclein (red) and chicken polylclonal antibody to MAP2 NB300-213 (green). The alpha-s

Specifications

Product Data

• Clone Name: 2A7
• Applications: IF, WB
• Recommended Dilution: WB: 1:1000, IF: 1:1000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human alpha-Synuclein [UniProt# P37840]
• Formulation: Preservative: 0.05% Sodium Azide. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Ascites
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 14.5 kDa
• Gene Name: synuclein alpha
• Database Link:
  NP_000336
  Entrez Gene 20617 MouseEntrez Gene 29219 RatEntrez Gene 6622 Human
  UniProt ID P37840
• Background: alpha-Synuclein (SNCA, NACP or PARK-1) is a neuronal protein normally localized to presynaptic terminals and has been involved in processes such as dopamine biosynthesis, synaptic plasticity, and vesicle dynamics. SNCA-VAMP2 direct binding promotes SNARE complex assembly through a nonclassical chaperone activity and regulation of SNARE complex's assembly/disassembly is essential for efficient neurotransmission. CSP-alpha, a presynaptic protein also promotes SNARE complex assembly via formation of chaperone complex with heat shock cognate 70 (Hsc70) and small glutamine-rich protein SGT (CSP-alpha-Hsc70-SGT complex). Interestingly, the CSP-alpha-knockout mice depicted rapidly progressive neurodegeneration followed by premature death which was counteracted by transgenic expression of SNCA. Overexpressed SNCA indirectly inhibited SNARE-mediated exocytosis by sequestering arachidonic acid, which upregulates syntaxin and enhances its engagement with SNARE complex. Tight regulation of subcellular level and distribution of SNCA is indispensable for the intrinsic functions of neuronal cells and genetic alterations of SNCA resulting in aberrant polymerization into fibrils are associated with several neurodegenerative diseases (synucleinopathies) including Parkinson disease type -1 (PARK1) - 4 (PARK4) as well as dementia Lewy body (DLB).
• Synonyms: NACP; PARK1; PARK4; PD1
• Note: This alpha-Synuclein antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot.

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Parkinson's disease