LCAT Rabbit Polyclonal Antibody

CAT#: TA336717

Rabbit Polyclonal LCAT Antibody

Product Images

Western Blot: LCAT Antibody TA336717 - Analysis of plasma LCAT protein mass. Plasma samples (0.2ul) from four hA-I Tg mice, four hA-I Tg SR-BI -/- mice, a LCAT-/- mouse, and purified mouse LCAT were fractionated on SDS-PAGE, and LCAT was detected by Western blot analysis using rabbit antiserum against mouse LCAT.

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  Immunocytochemistry/Immunofluorescence: LCAT Antibody TA336717 - The LCAT antibody was tested at a 1:500 dilution in HeLa cells against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue).

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western Blot: 1:1000, Immunocytochemistry/ Immunofluorescence: 1:500
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Carboxyl-terminal histidine-tagged mouse recombinant LCAT purified from the medium of CHO cells overexpressing mouse LCAT cDNA. [UniProt# P16301]
• Formulation: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Whole antisera
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: lecithin-cholesterol acyltransferase
• Database Link:
  NP_000220
  Entrez Gene 16816 MouseEntrez Gene 3931 Human
  UniProt ID P04180
• Background: Lecithin: cholesterol acyltransferase (LCAT) is the plasma enzyme responsible for most HDL cholesteryl esters in human plasma. In species with cholesteryl ester transfer protein (CETP) it is also responsible for a significant proportion of VLDL and LDL cholesteryl esters. LCAT, through the esterification of cholesterol, plays a role in the reverse cholesterol transport pathway by facilitating the net movement of cholesterol from peripheral tissues back to the liver for excretion. LCAT deficiency results in low levels of plasma HDL, whereas a transgenic overexpression of LCAT results in increased plasma HDL concentrations.
• Synonyms: lecithin-cholesterol acyltransferase
• Note: This LCAT antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen at ~62-65 kDa representing LCAT. Also has been shown to immunodeplete LCAT from mouse plasma, suggesting it should work fine for Immunoprecipitation (PMID: 18583707).

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Glycerophospholipid metabolism