p95 NBS1 (NBN) Rabbit Polyclonal Antibody

CAT#: TA336720

Rabbit Polyclonal Nbs1 Antibody

Product Images

Western Blot: Nbs1 Antibody TA336720 - Analysis of Nbs1 in NIH/3T3 (Lane 4) and HeLa whole cell extract (Lane 5) with Nbs1 Antibody TA336720. Observed molecular weight at ~99 kDa.

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  Immunohistochemistry-Paraffin: Nbs1 Antibody TA336720 - Staining of paraffin-embedded mouse bone marrow using Nbs1 Antibody TA336720.

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  Immunohistochemical staining of MRE11, RAD50 and NBS1 proteins. Staining for each protein was scored as high or low as described in the Methods section. Representative examples of typical nuclear staining of MRE11 (a), RAD50 (b), and NBS1(c) scored as high expression in tumor cells. Correspondingly, examples of those scored as low expression for MRE11 (d), RAD50 (e), and NBS1 (f) are shown (40× magnification)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Immunohistochemistry-Paraffin, Western Blot: 2 ug/mL, Immunohistochemistry: 2 ug/mL
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide made to an internal portion of the mouse NBS1 protein (within residues 350-400). [Swiss-Prot# Q9R207]
• Formulation: PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 97 kDa
• Gene Name: nibrin
• Database Link:
  NP_002476
  Entrez Gene 27354 MouseEntrez Gene 4683 Human
  UniProt ID O60934
• Background: NBS1 (Nijmegen breakage syndrome protein 1) is a component of MRN complex (Mre11-Rad50-Nbs1) that plays important role in detection and signaling of DNA double strand breaks (DSBs) through acting as DSB sensor, co-activator of DSB-induced cell cycle checkpoint signaling, and as repair-effector in two competing DSB repair pathways: homologous recombination (HR) and non-homologous end-joining (NHEJ). MRN complex also associates with telomeres at the ends of linear chromosomes, where it contributes to their maintenance. NBS1's FHA domain binds phospho-Thr residues in Ser-X-Thr motifs present in DNA damage proteins, including Mdc1 and Ctp1, whereas, BRCT domains of NBS1 bind Ser-X-Thr motifs when Ser residue is phosphorylated. These phospho-dependent interactions are important for recruiting repair and checkpoint proteins to DSB sites and NBS1 is responsible for MRN complex's nuclear translocation. NBS1 itself does not possess enzymatic activity and contributes to DSB repair primarily by mediating protein-protein interactions at DNA breakage sites. The central region of NBS1 1 possesses several SQ motifs that are phosphorylated by ATM kinase via DNA damage response. NBS1's C-terminus contains a domain that interacts with ATM and recruits it to DSBs, and induces apoptosis in response to damage. DSBs can be caused by ionizing radiation, certain chemotherapy drugs, metabolic ROS, as errors during replication, by programmed enzymatic activities during meiosis/V(D)J recombination etc., and if left unrepaired, DSBs can generate chromosomal translocations, aneuploidy and carcinogenesis.
• Synonyms: AT-V1; AT-V2; ATV; NBS; NBS1; P95
• Note: This NBS1 antibody is useful for Immunohistochemistry on paraffin embedded sections and Western blot, where a band is seen at ~97 kDa.

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Homologous recombination