MCP1 (CCL2) Rabbit Polyclonal Antibody

CAT#: TA336724

Rabbit Polyclonal CCL2/MCP1 Antibody

Product Images

Immunocytochemistry/Immunofluorescence: CCL2/MCP1 Antibody TA336724 - MCP1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

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  Western Blot: CCL2/MCP1 Antibody TA336724 - CCL2 expression level was evaluated in control group and aLAG-3 group.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western Blot: 1:1000, Block/Neutralize, Immunocytochemistry/ Immunofluorescence: 1:2000, Immunohistochemistry: 1:500-1:1000, Immunohistochemistry-Paraffin: 1:500-1:1000
• Reactivities: Human, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide made to an internal portion of rat MCP1 (within residues 15-40). [Swiss-Prot# P14844]
• Formulation: PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 16 kDa
• Gene Name: C-C motif chemokine ligand 2
• Database Link:
  NP_002973
  Entrez Gene 24770 RatEntrez Gene 6347 Human
  UniProt ID P13500
• Background: MCP-1 (monocyte chemoattracctant protein-1, also called C-C motif chemokine 2 or CCL2) is a secreted protein belonging to intercrine beta (chemokine CC) family and it specifically act as a chemotactic factor to attracts monocytes, CD4+ T cells, NK cells as well as for CCR2 expressing cells, but not neutrophils. Both hematopoietic and non-hematopoietic cells that include monocytes/macrophages, dendritic cells, astrocytes, endothelial cells and fibroblasts express MCP-1, and monocytes/macrophages are the major source of MCP-1 in peripheral blood. Besides, expression at acute inflammation sites, MCP-1 is upregulated in chronic inflammation, including autoimmune disease, atherosclerosis, cancer, and chronic infection. MCP-1 has been implicated in both pro-inflammatory and anti-inflammatory responses, and is a potential intervention point for the treatment of various inflammatory/autoimmune diseases (multiple sclerosis, atherosclerosis, insulin-resistant diabetes, rheumatoid arthritis and lupus nephritis), cancers (breast and prostate cancer) and HIV-1/AIDS.
• Synonyms: GDCF-2; HC11; HSMCR30; MCAF; MCP-1; MCP1; SCYA2; SMC-CF
• Note: This MCP1 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen at ~16 kDa. In ICC/IP secretory vesicles staining was observed in HeLa cells. IHC-Fr and B/N applications are reported in research publications with PMIDs 22402584 and 22778093 respectively.

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Chemokine signaling pathway, Cytokine-cytokine receptor interaction, NOD-like receptor signaling pathway