AMBRA1 Rabbit Polyclonal Antibody

CAT#: TA336728

Rabbit Polyclonal AMBRA1 Antibody

Product Images

Immunohistochemistry: AMBRA1 Antibody TA336728 - IHC analysis of AMBRA1 in mouse brain using DAB with hematoxylin counterstain.

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  Immunocytochemistry/Immunofluorescence: AMBRA1 Antibody TA336728 - AMBRA1 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

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  Immunohistochemistry: AMBRA1 Antibody TA336728 - IHC analysis of AMBRA1 in mouse epidermis using DAB with hematoxylin counterstain.

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  Western Blot: AMBRA1 Antibody TA336728 - WB analysis of AMBRA1 in mouse liver cell lysate.

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  The protein levels of AMBRA1, LC3 and SQSTM1 were analyzed by western blot from fresh tissue samples. Tubulin was also detected as the control of sample loading. Representative western blots were shown. N normal tissue, T PCa tissue.

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Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1:100-1:2000, Immunohistochemistry: 1:400, Immunocytochemistry/ Immunofluorescence: 1:100, Western Blot: 1:1000, Immunohistochemistry-Paraffin: 1:400
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A synthetic peptide derived from mouse activating molecule in Beclin 1-regulated/AMBRA1 protein sequence [amino acids range 1175-1250] [UniProt # A2AH22]
• Formulation: Tris-citrate/phosphate, pH 7, 0.1% Sodium azide. Store at 4C. Do not freeze.
• Concentration: lot specific
• Purification: Affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: autophagy and beclin 1 regulator 1
• Database Link:
  NP_060219
  Entrez Gene 59319 RatEntrez Gene 228361 MouseEntrez Gene 55626 Human
  UniProt ID Q9C0C7
• Background: AMBRA1 (Activating molecule in Beclin1-regulated autophagy protein 1, Kiaa1736, DCAF3) is a regulator of autophagy and neural development in embryogenesis. Ambra1 controls autophagy in the central nervous system by activating class III PtdIns3k complexes that are required for phagophore nucleation. Beclin1 and Vps34 interaction are promoted by AMBRA1, and help form this complex. The loss of AMBRA1 causes upregulated apoptosis and accumulation of ubiquitinated proteins, which finally proves lethal. It was recently discovered that AMBRA1 interacts with Parkin after prolonged mitochondrial depolarization, thus triggering mitophagy (PMID 21921694). AMBRA1 has 7 known isoforms, and numerous WD40 domains.
• Synonyms: DCAF3; WDR94
• Note: This AMBRA1 antibody is useful for Western Blot, Immunocytochemistry/Immunofluorescence, and IHC-paraffin embedded sections. In Western Blot, a band is seen ~ 142 kDa representing AMBRA1. In ICC/IF, cytoplasmic staining was observed in HeLa cells. In IHC-P, staining was observed compartmentalized in cytoplasmic vesicles of mouse brain and skin tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.