PAI1 (SERPINE1) Rabbit Polyclonal Antibody

CAT#: TA336741

Rabbit Polyclonal Serpin E1/PAI-1 Antibody

Product Images

Immunohistochemistry-Paraffin: Serpin E1/PAI-1 Antibody TA336741 - Staining of PAI1/Serpine 1 in mouse pancreas.

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  Immunocytochemistry/Immunofluorescence: Serpin E1/PAI-1 Antibody TA336741 - PAI1/Serpine1 antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

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  Western Blot: Serpin E1/PAI-1 Antibody TA336741 - Extracts from Jurkat cells.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Immunocytochemistry/ Immunofluorescence: 5 - 10 ug/ml, Immunohistochemistry: 1:50 - 1:100, Immunohistochemistry-Frozen: 1:50 - 1:100, Immunohistochemistry-Paraffin: 1:50 - 1:100, Western Blot: 1.0 ug/ml, ELISA
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A synthetic peptide made to an internal portion of the human PAI1/Serpine 1 protein (between residues 300-400) [UniProt P05121]
• Formulation: PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 45 kDa
• Gene Name: serpin family E member 1
• Database Link:
  NP_000593
  Entrez Gene 18787 MouseEntrez Gene 24617 RatEntrez Gene 5054 Human
  UniProt ID P05121
• Background: PAI-1 and PAI-2 (for plasminogen activator inhibitor-1 and -2) are members of the serpin serine proteinase inhibitor family. PAI-1 and PAI-2 have been shown to regulate uPA (urokinase-type plasminogen activator) and tPA (tissue plasminogen activator), resulting in the inhibition of proteolytic activity. Members of the serpin family generally complex with their target proteinases, then disassociate slowly into cleaved species that fold into stable inactive forms. PAI-1 can fold into the inactive state without cleavage, resulting in the latent form of PAI-1. Activity can be restored to the latent form of PAI-1 through denaturation and renaturation. PAI-2 occurs in secreted and cytosolic forms through facultative polypeptide translocation. uPA is a serine proteinase that is a member of the trypsin family. It is responsible for the cleavage of plasminogen at the Arg-Val bond to produce plasmin. uPA consists of two chains designated A and B. The A chain can be cleaved, resulting in low and high molecular mass forms of uPA.
• Synonyms: PAI; PAI-1; PAI1; PLANH1

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Complement and coagulation cascades, p53 signaling pathway