CDCA8 Rabbit Polyclonal Antibody

CAT#: TA336746

Rabbit Polyclonal Borealin Antibody

Product Images

Western Blot: Borealin Antibody TA336746 - Mouse kidney lysate.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western Blot: 2 ug/ml, Immunocytochemistry/ Immunofluorescence: 1:50-1:200
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide made to an internal portion of human Borealin (within residues 200-250). [Swiss-Prot# Q53HL2]
• Formulation: PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 38 kDa
• Gene Name: cell division cycle associated 8
• Database Link:
  NP_060571
  Entrez Gene 55143 Human
  UniProt ID Q53HL2
• Background: Encoded by gene CDCA8, borealin forms an important component of mitosis regulating chromosomal passenger complex (CPC complex - BIRC5/survivin, CDCA8/borealin, INCENP, AURKB and AURKC) which is known for ensuring correct chromosome alignment/segregation and is required for chromatin-induced microtubule stabilization as well as spindle assembly. Borealin governs CPC to centromeric DNA and is a major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment. Besides associating with CPC complex subunits, borealin interact with SENP3, UBE2I and RANBP2 which are involved its sumoylation. Borealin is expressed in a cell cycle-dependent manner and the levels of endogenous borealin significantly increase during mitosis. TTK can phosphorylated borealin at Thr-88, Thr94, Thr-169 and Thr-230 and and mutation of S219 to alanine in borealin causes defects in mitotic progression advocating for the importance of phosphorylation in borealin function. Mutation of additional sites creates a form of borealin that is defective in localizing to the centromere, binding to Sgo1 and Sgo2, and in coordinating chromosome alignment.
• Synonyms: BOR; BOREALIN; DasraB; MESRGP
• Note: This Borealin antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band can be seen ~38 kDa.

Reference Data

• Protein Families: Druggable Genome