GAPDH Mouse Monoclonal Antibody [Clone ID: 1A10]

CAT#: TA336768

Mouse Monoclonal GAPDH/G3PDH Antibody (1A10)


USD 424.00

5 Days*

Size
    • 100 ul

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Specifications

Product Data
Clone Name 1A10
Applications IHC, WB
Recommended Dilution Flow Cytometry: 5 ug/ml, Immunohistochemistry-Paraffin: 1:200-1:1000, Western Blot: 1:500-1:2000, ELISA: 1:10000, Immunohistochemistry: 1:200-1:1000, Immunocytochemistry/ Immunofluorescence: 1:200-1:1000, Simple Western: 1:50
Reactivities Human
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Purified recombinant fragment of human GAPDH expressed in E. coli. [UniProt# P04406]
Formulation PBS, 0.03% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration lot specific
Purification Ammonium sulfate precipitation
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Predicted Protein Size 37 kDa
Gene Name glyceraldehyde-3-phosphate dehydrogenase
Background GAPDH is a 146 kDa tetramer composed of four 30-40 kDa subunits. Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate. Because GAPDH as a protein expressed in large amounts and which is required at all times for an important house keeping functions, levels of GAPDH mRNA are often used as standards in studies of mRNA expression. Increasingly, scientists are making use of specific antibodies to GAPDH as loading controls for western blotting experiments. Apart from a role in glycolysis, GAPDH may have other roles such as in the activation of transcription. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease, and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations with actin and tubulin have also be reported. The protein may also have a role in the regulation of apoptosis, and interestingly migrates from the cytoplasm into the nucleus when cells become apoptotic.
Synonyms G3PD; GAPD; HEL-S-162eP
Note This GAPDH (1A10) antibody is useful for Western blot, Immunohistochemistry on paraffin embedded sections, Immunocytochemistry/Immunofluorescence and ELISA.
Reference Data
Protein Families ES Cell Differentiation/IPS
Protein Pathways Alzheimer's disease, Glycolysis / Gluconeogenesis, Metabolic pathways

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.