GSK3 beta (GSK3B) Mouse Monoclonal Antibody [Clone ID: 3D10]

CAT#: TA336772

Mouse Monoclonal GSK-3 beta Antibody (3D10)

Product Images

Western Blot: GSK-3 beta Antibody (3D10) TA336772 - Analysis using GSK3 beta mouse mAb against A549 (1), K562 (2), PC-12 (3), NIH/3T3 (4), and HEK293 (5) cell lysates.

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  Immunohistochemistry-Paraffin: GSK-3 beta Antibody (3D10) TA336772 - Analysis of paraffin-embedded human lung cancer (left) and breast cancer tissues (right) using GSK3 beta mouse mAb with DAB staining.

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  Simple Western: GSK-3 beta Antibody (3D10) TA336772 - Simple Western lane view shows a specific band for GSK-3 Beta in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

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  Immunocytochemistry/Immunofluorescence: GSK-3 beta Antibody (3D10) TA336772 - Analysis of NIH/3T3 (left) and U251 (right) cells using GSK3 beta mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

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  Flow Cytometry: GSK-3 beta Antibody (3D10) TA336772 - Flow cytometric analysis of Hela cells using GSK3 beta mouse mAb (green) and negative control (purple).

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  Flow Cytometry: GSK-3 beta Antibody (3D10) TA336772 - An intracellular stain was performed on HeLa cells with GSK-3 beta (3D10) antibody TA336772PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.

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Specifications

Product Data

• Clone Name: 3D10
• Applications: FC, IHC, WB
• Recommended Dilution: Immunocytochemistry/ Immunofluorescence: 1:200-1:1000, Western Blot: 1:500-1:2000, Immunohistochemistry-Paraffin: 1:200-1:1000, Simple Western: 1:50, Flow Cytometry: 1:200-1:400, ELISA: 1:10000, Immunohistochemistry: 1:200-1:1000
• Reactivities: Human, Mouse, Rat, Primate
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of human GSK3 beta expressed in E. coli. [UniProt# P49841]
• Formulation: PBS, 0.03% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Ammonium sulfate precipitation
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 46 kDa
• Gene Name: glycogen synthase kinase 3 beta
• Database Link:
  NP_002084
  Entrez Gene 56637 MouseEntrez Gene 84027 RatEntrez Gene 2932 Human
  UniProt ID P49841
• Background: GSK3 (Glycogen synthase kinase 3), a serine-threonine kinase with two isoforms (alpha and beta), was originally discovered as a key enzyme in glycogen metabolism. GSK3 was subsequently shown to function in cell division, proliferation, motility and survival. GSK3 plays a role in a number of pathological conditions including cancer and diabetes and is increasingly seen as an important component of neurological diseases. GSK3 phosphorylates tau and presenilin-1, which are involved in the development of Alzheimer's disease. Both isoforms of GSK3 are ubiquitously expressed, although particularly high levels of GSK 3 beta are found in the brain where it is involved in synaptic plasticity, possibly via regulation of NMDA receptor trafficking. GSK3 phosphorylates over 40 different substrates including signaling proteins, transcription factors and structural proteins, and is part of the signal transduction cascade of a large number of growth factors and cytokines. The activity of GSK is regulated by phosphorylation (Akt: Akt-mediated phosphorylation at Ser21 of GSK3 alpha and Ser9 of GSK3 beta, S6K, RSK, PKA and PKC), dephosphorylation (PP1 and PP2A), and by binding to protein complexes (with beta-catenin, axin, CK1 and the APC complex).
• Synonyms: glycogen synthase kinase-3 beta; glycogen synthase kinase 3 beta; GSK3beta isoform
• Note: This GSK3 beta (3D10) antibody is useful for Western blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and ELISA.

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Alzheimer's disease, Axon guidance, Basal cell carcinoma, B cell receptor signaling pathway, Cell cycle, Chemokine signaling pathway, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Focal adhesion, Hedgehog signaling pathway, Insulin signaling pathway, Melanogenesis, Neurotrophin signaling pathway, Pathways in cancer, Prostate cancer, T cell receptor signaling pathway, Wnt signaling pathway