VCAM1 Mouse Monoclonal Antibody [Clone ID: 6G9]

CAT#: TA336775

Mouse Monoclonal VCAM-1/CD106 Antibody (6G9)

Product Images

Western Blot: VCAM-1/CD106 Antibody (6G9) TA336775 - Using VCAM1 mouse mAb against (1) HUVEC and (2) EC cell lysate.

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  Simple Western: VCAM-1/CD106 Antibody (6G9) TA336775 - Lane view shows a specific band for CD106/VCAM1 in 0.5 mg/mL of HUVEC lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

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  Photomicrographs of two separate gut sections from a patient with EHEC colitis.Panels (A) and (B) are stained with CD31 to enumerate endothelium lining the vessels (40× magnification). (C) and (D) are stained to show VCAM-1 expression in endothelium, indicating inflammatory activation (40× magnification).

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  Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (6G9) TA336775 - Human placenta tissues using VCAM1 mouse mAb.

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  Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (6G9) TA336775 - (A) human lung cancer and (B) colon cancer using VCAM1 mouse mAb with DAB staining.

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Specifications

Product Data

• Clone Name: 6G9
• Applications: IHC, WB
• Recommended Dilution: Simple Western: 1:1000, ELISA: 1:10000, Immunoprecipitation, Immunoblotting, Western Blot: 1:500-1:2000, Immunohistochemistry: 1:200-1:1000, Immunohistochemistry-Paraffin: 1:200-1:1000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of human VCAM1 expressed in E. coli. [UniProt# P19320]
• Formulation: PBS, 0.03% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Ammonium sulfate precipitation
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 81 kDa
• Gene Name: vascular cell adhesion molecule 1
• Database Link:
  NP_001069
  Entrez Gene 7412 Human
  UniProt ID P19320
• Background: VCAM1 is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Two alternatively spliced transcripts encoding different isoforms have been described for this gene. Tissue specificity: Expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue.
• Synonyms: CD106; INCAM-100
• Note: This VCAM1 (6G9) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections and ELISA.

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), Leukocyte transendothelial migration