KDM3A / JHDM2A (KDM3A) Rabbit Polyclonal Antibody

CAT#: TA336794

Rabbit Polyclonal Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody


USD 424.00

5 Days*

Size
    • 100 ul

Product Images

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Specifications

Product Data
Applications IHC, WB
Recommended Dilution Immunohistochemistry: 1:50, Immunocytochemistry/ Immunofluorescence: 1:500-1:2000, Western Blot: 1:5000, Immunohistochemistry-Paraffin: 1:50
Reactivities Human, Mouse, Primate
Host Rabbit
Clonality Polyclonal
Immunogen A genomic peptide made to an internal region of the human JMJD1A protein (within residues 250-400). [Swiss-Prot Q9Y4C1]
Formulation PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration lot specific
Purification Immunogen affinity purified
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Gene Name lysine demethylase 3A
Background JMJD1A is a histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. It preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. JMJD1A is involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. It's also Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1.
Synonyms JHDM2A; JHMD2A; JMJD1; JMJD1A; TSGA
Note This JMJD1A antibody is useful for immunohistochemistry on paraffin tissues, immunocytochemistry/immunofluorescence and Western blot where a band is seen ~147 kDa. In ICC/IF, nuclear signal was observed in MCF-7 cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.