eNOS (NOS3) Mouse Monoclonal Antibody [Clone ID: 6H2]

CAT#: TA336799

Mouse Monoclonal eNOS Antibody (6H2)

Product Images

Immunohistochemistry-Paraffin: eNOS Antibody (6H2) TA336799 - IHC staining of eNOS in human placenta using DAB with hematoxylin counterstain.

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  Immunohistochemistry-Paraffin: eNOS Antibody (6H2) TA336799 - Immunohistochemical analysis of paraffin-embedded human lymph node (left) and colon cancer (right) tissues using eNOS mouse mAb with DAB staining.

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  Immunohistochemistry-Paraffin: eNOS Antibody (6H2) TA336799 - Immunohistochemical analysis of paraffin-embedded human stomach cancer (left) and ovary cancer (right) tissues using eNOS mouse mAb with DAB staining.

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  Western Blot: eNOS Antibody (6H2) TA336799 - Western blot analysis of HUVEC cells (A) using eNOS antibody at a concentration of 2 ug/ml.

Specifications

Product Data

• Clone Name: 6H2
• Applications: IHC, WB
• Recommended Dilution: Immunohistochemistry-Paraffin: 1:100, Immunohistochemistry: 1:100, Western Blot: 2 ug/ml, ELISA: 1:10000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of human eNOS expressed in E. coli. [UniProt# P29474]
• Formulation: PBS, 0.03% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Ammonium sulfate precipitation
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 133 kDa
• Gene Name: nitric oxide synthase 3
• Database Link:
  NP_000594
  Entrez Gene 4846 Human
  UniProt ID P29474
• Background: eNOS (Endothelial nitric-oxide synthase), also known as NOS3, it is an important enzyme in the cardiovascular system. It is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm.
• Synonyms: ECNOS; eNOS
• Note: This eNOS (6H2) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections and ELISA.

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Arginine and proline metabolism, Calcium signaling pathway, Long-term depression, Metabolic pathways, Pathways in cancer, Small cell lung cancer, VEGF signaling pathway