p75 NGF Receptor (NGFR) Mouse Monoclonal Antibody [Clone ID: 2F1C2]

CAT#: TA336800

Mouse Monoclonal p75NTR/NGF Receptor Antibody (2F1C2)

Product Images

Flow Cytometry: NGF R/TNFRSF16/p75NTR Antibody (2F1C2) TA336800 - Analysis of EC cells using p75NTR/NGF Receptor mouse mAb (green) and negative control (purple).

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  Immunocytochemistry/Immunofluorescence: NGF R/TNFRSF16/p75NTR Antibody (2F1C2) TA336800 - Analysis of EC cells using p75NTR/NGF Receptor mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

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  Western Blot: NGF R/TNFRSF16/p75NTR Antibody (2F1C2) TA336800 - Analysis using p75NTR/NGF Receptor mouse mAb against NGFR-hIgGFc transfected HEK293 cell lysate.

Specifications

Product Data

• Clone Name: 2F1C2
• Applications: FC, WB
• Recommended Dilution: ELISA: 1:10000, Western Blot: 1:500 - 1:2000, Flow Cytometry: 1:200 - 1:400, Immunocytochemistry/ Immunofluorescence: 1:200 - 1:1000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of human p75NTR/NGF Receptor expressed in E. coli. [UniProt# P08138]
• Formulation: Preservative: 0.05% Sodium Azide. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Ascites
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 45 kDa
• Gene Name: nerve growth factor receptor
• Database Link:
  NP_002498
  Entrez Gene 4804 Human
  UniProt ID P08138
• Background: p75NTR/NGF Receptor, also known as Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. p75NTR/NGF Receptor plays a central role in the regulation of cell number by apoptosis in the developing CNS. During early development, activation of p75NTR/NGF Receptor by NGF induces apoptotic cell death in some neuronal cells, probably through activation of the sphingomyelinase/ceramide pathway, the ICE like proteases and the JNK pathway. In rat Schwann cells, NGF binding to p75NTR/NGF Receptor activates NF kappaB, possibly to modulate Schwann cell migration during nerve regeneration. CD271 has recently been described as being expressed in mesenchymal stem cells (bone marrow stromal cells).
• Synonyms: CD271; Gp80-LNGFR; p75(NTR); p75NTR; TNFRSF16
• Note: This p75NTR/NGF Receptor (2F1C2) antibody is useful in Western Blot, ELISA, Immunocytochemistry/Immunofluorescence and Flow Cytometry.

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction, Neurotrophin signaling pathway