Oct4 (POU5F1) Mouse Monoclonal Antibody [Clone ID: 7E7]

CAT#: TA336803

Mouse Monoclonal OCT4 Antibody (7E7)

Product Images

Flow Cytometry: OCT4 Antibody (7E7) TA336803 - Flow cytometric analysis of Jurket cells using OCT4 mouse mAb (green) and negative control (purple).

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  Immunocytochemistry/Immunofluorescence: OCT4 Antibody (7E7) TA336803 - Analysis of NTERA-2 cells using OCT4 mouse mAb (green). Actin filaments have been labeled with Alexa Fluor-555 phalloidin (red).

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  Immunocytochemistry/Immunofluorescence: OCT4 Antibody (7E7) TA336803 - Analysis of NTERA-2 cells using OCT4 mouse mAb (green). Actin filaments have been labeled with Alexa Fluor-555 phalloidin (red).

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  Western Blot: OCT4 Antibody (7E7) TA336803 - Analysis using OCT4 mouse mAb against NTERA-2 cell lysate.

Specifications

Product Data

• Clone Name: 7E7
• Applications: FC, WB
• Recommended Dilution: Flow Cytometry: 1:200-1:400, ELISA: 1:10000, Immunocytochemistry/ Immunofluorescence: 1:200-1:1000, Western Blot: 1:500-1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic peptide derived from an internal portion of human OCT4. [UniProt# Q01860]
• Formulation: Preservative: 0.05% Sodium Azide. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Ascites
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 45 kDa
• Gene Name: POU class 5 homeobox 1
• Database Link:
  NP_002692
  Entrez Gene 5460 Human
  UniProt ID Q01860
• Background: The OCT4 gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. (provided by RefSeq).Tissue specificity: Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
• Synonyms: Oct-3; Oct-4; OCT3; OCT4; OTF-3; OTF3; OTF4
• Note: This OCT4 (7E7) antibody is useful for Western Blot, ELISA, Flow Cytometry and Immunocytochemistry/Immunofluorescence.

Reference Data

• Protein Families: Adult stem cells, Cancer stem cells, Embryonic stem cells, Induced pluripotent stem cells, Stem cell - Pluripotency, Transcription Factors