Stathmin 1 (STMN1) Rabbit Polyclonal Antibody

CAT#: TA336822

Rabbit Polyclonal Stathmin 1 Antibody

Product Images

Western Blot: Stathmin 1 Antibody TA336822 - EL4 cell lysate with op18 antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL.

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  Immunocytochemistry/Immunofluorescence: Stathmin 1 Antibody TA336822 - Stathmin 1 antibody was tested in PC12 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

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  Knockout Validated: Stathmin 1 Antibody TA336822 - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Stathmin 1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human SIRT1 Polyclonal Antibody (Catalog # TA336822) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Stathmin 1 at approximately 17 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.

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  Immunohistochemistry-Paraffin: Stathmin 1 Antibody TA336822 - Human brain tissue with op18 antibody at 2.5 ug/ml.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Immunocytochemistry/ Immunofluorescence, Knockout Validated, Immunohistochemistry: 1:100 - 1:250, Western Blot: 0.5-1 ug/ml, Immunohistochemistry-Paraffin: 1:100 - 1:250, ELISA: 1:100-1:2000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A synthetic peptide made to an C-terminal portion of the human Stathmin 1 protein (between residues 100-149) [UniProt P16949]
• Formulation: PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 17 kDa
• Gene Name: stathmin 1
• Database Link:
  NP_981944
  Entrez Gene 16765 MouseEntrez Gene 29332 RatEntrez Gene 3925 Human
  UniProt ID P16949
• Background: Stathmin 1 (STMN1), also known as leukemia-associated phosphoprotein p18, metablastin, oncoprotein 18 (op18), phosphoprotein p19, prosolin, protein Pr22, and pp17, is a member of the stathmin family that encodes a ubiquitous cytosolic phosphoprotein that functions as an intracellular relay for regulatory signals of the cellular environment. STMN1 is involved in the regulation of the microtubule filament system by destabilizing microtubules. In addition, STMN1 has resently been implicated as a potential target of the ASK1-p38 MAP kinase cascade. Phosphorylation at STMN1 Ser-16 may be required for axon formation during neurogenesis. The STMN1 gene has been linked to the control of the learned and innate fear responses. STMN1 is highly expressed in a wide variety of human malignancies, including leukemia, prostate cancer, ovarian carcinoma, and breast carcinoma; suggesting that STMN1 may be an ideal target for anti-cancer therapeutics.
• Synonyms: C1orf215; Lag; LAP18; OP18; PP17; PP19; PR22; SMN

Reference Data

• Protein Pathways: MAPK signaling pathway