BNIP3L Rabbit Polyclonal Antibody

CAT#: TA336844

Rabbit Polyclonal BNIP3L Antibody

Product Images

Immunocytochemistry/Immunofluorescence: BNIP3L Antibody TA336844 - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-BNIP3L (TA336844) at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with and anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

• 

  Immunohistochemistry: BNIP3L Antibody TA336844 - Analysis of BNIP3L in mouse prostate using DAB with hematoxylin counterstain.

Specifications

Product Data

• Applications: IHC
• Recommended Dilution: Immunohistochemistry: 1:300, Immunocytochemistry/ Immunofluorescence: 1:100-1:1000, Immunohistochemistry-Paraffin: 1:300
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A synthetic peptide made to an internal portion of the human BNIP3L protein (between residues 50-100) [UniProt O60238]
• Formulation: PBS with 30% Glycerol, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: BCL2/adenovirus E1B 19kDa interacting protein 3-like
• Database Link:
  NP_004322
  Entrez Gene 12177 MouseEntrez Gene 665 Human
  UniProt ID O60238
• Background: BNIP3L/NIX is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family, which is involved in the regulation of apoptosis. Like other BH3-only proteins, NIX is believed to possess tumor suppressing functions; NIX localizes to the mitochondria and interacts with BCL2 and BCL-X in a pro-apoptotic manner. NIX shares a high level of sequence homology with BNIP3, and like BNIP3 activates apoptosis through its C-terminal transmembrane domain but not via its BH3 domain. Hypoxia is believed to be a principle regulator of NIX activity, making NIX a key factor in hypoxia-induced tumor cell death and heart disease. In addition, NIX has been implicated in mitophagy, a specific form of autophagy; due to its dual role, NIX is an interesting target to examine the relationship between apoptosis and autophagy.
• Synonyms: BNIP3a; NIX
• Note: This BNIP3L antibody is useful for Immunocytochemistry/Immunofluorescence and Immunohistochemistry-Paraffin. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Reference Data

• Protein Families: Druggable Genome, Transmembrane