TMS1 (PYCARD) Rabbit Polyclonal Antibody

CAT#: TA336851

Rabbit Polyclonal ASC/TMS1 Antibody

Product Images

Immunocytochemistry/Immunofluorescence: ASC/TMS1 Antibody TA336851 - Tested in A431 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).

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  Immunohistochemistry: ASC/TMS1 Antibody TA336851 - Analysis of ASC/TMS1 in mouse intestine using DAB with hematoxylin counterstain.

Specifications

Product Data

• Applications: IHC
• Recommended Dilution: Immunohistochemistry: 1:200, Immunocytochemistry/ Immunofluorescence: 1:40-1:100, Immunohistochemistry-Paraffin: 1:200, Western Blot (Negative)
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A synthetic peptide made to a C-terminal portion of the human ASC/TMS1 protein (between residues 145-195) [UniProt Q9ULZ3]
• Formulation: PBS with 30% Glycerol, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: PYD and CARD domain containing
• Database Link:
  NP_037390
  Entrez Gene 66824 MouseEntrez Gene 29108 Human
  UniProt ID Q9ULZ3
• Background: Target of methylation-induced silencing 1 protein, TMS1 (ASC, Pycard) is a 21 kDa cytoplasmic adaptor protein containing 2 protein-protein interaction domains: an N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). Both domains are members of the six-helix bundle death domain superfamily. These domains, via caspase activation, mediate the assembly of large signaling complexes that are involved in the apoptotic and inflammatory signaling pathways. During apoptosis TMS1 aggregates near the nuclear periphery as ball-like structures. Recent research suggests that it can modulate MAPK expression by suppressing the DUSP10/MKP5 phosphatase thus allowing the phosphorylation of MAPK by pathogens and toll-like receptor agonists. PMID: 2148701.
• Synonyms: ASC; CARD5; TMS; TMS-1; TMS1
• Note: This ASC/TMS1 antibody is useful for Immunocytochemistry/Immunofluorescence and IHC-paraffin embedded sections. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. This antibody is not recommended for Western Blot.

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Cytosolic DNA-sensing pathway, NOD-like receptor signaling pathway