CARD12 (NLRC4) Rabbit Polyclonal Antibody

CAT#: TA336852

Rabbit Polyclonal CARD12 Antibody

Product Images

Immunocytochemistry/Immunofluorescence: CARD12 Antibody TA336852 - The CARD12 antibody (TA336852) was tested in HepG2 cells at a 1:250 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.

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  Immunohistochemistry: CARD12 Antibody TA336852 - IHC analysis of CARD12 in mouse lung using DAB with hematoxylin counterstain.

Specifications

Product Data

• Applications: IHC
• Recommended Dilution: Immunoblotting, Flow Cytometry, Immunohistochemistry: 1:400, Immunocytochemistry/ Immunofluorescence: 1:100, Immunohistochemistry-Paraffin: 1:400
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A synthetic peptide made to an internal portion of the human CARD12 protein (between residues 600-700) [UniProt Q9NPP4]
• Formulation: PBS with 30% Glycerol, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: NLR family, CARD domain containing 4
• Database Link:
  NP_067032
  Entrez Gene 268973 MouseEntrez Gene 58484 Human
  UniProt ID Q9NPP4
• Background: CARD12, also known as CLAN, IPAF and CLAN1 is a member of the CED4 family of proteins. The CED4/Apaf-1 proteins are critical regulators of apoptosis and NF-kappaB signaling pathways, and CARD12 is no exception. CARD12 has been implicated in coordination of downstream activation of both apoptotic and inflammatory signaling pathways (PMID: 11374873). Additionally, CARD12 has shown to coprecipitate with caspase 1, an enzyme that participates in both the signaling of apoptosis and cytokine processing, further demonstrating its integral role in programmed cell death (PMID: 11374873). As part of the nucleotide-binding site and leucine-rich repeat (NBS-LRR) proteins, CARD12 is known to be an important regulator of caspase-1 activation via an ATP/dATP nucleotide-binding pocket (PMID: 15882992). CARD12 has four known isoforms. Isoform 1 is highly expressed in lung, followed by leukocytes (especially monocytes), lymph node, colon, brain, prostate, placenta, spleen, bone marrow and fetal liver. Although it is expressed ubiquitously, Isoform 2 is more prominent at the highest levels in lung and spleen. Isoform 4 can only been found in the brain.
• Synonyms: AIFEC; CARD12; CLAN; CLAN1; CLANA; CLANB; CLANC; CLAND; CLR2.1; FCAS4; IPAF
• Note: This CARD12 antibody is useful for Immunocytochemistry/Immunofluorescence and IHC-paraffin embedded sections. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Reference Data

• Protein Pathways: NOD-like receptor signaling pathway