Alpha Fodrin (SPTAN1) Rabbit Polyclonal Antibody

CAT#: TA336859

Rabbit Polyclonal Alpha Fodrin Antibody

Product Images

Western Blot: Alpha Fodrin Antibody TA336859 - Western blot of mouse sciatic nerve extract (lanes 1 and 2) and brain homogenate (3). A prominent band running at 240kDa represents the intact Alpha Fodrin heavy chain. More minor bands below this likely re

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  Immunocytochemistry/Immunofluorescence: Alpha Fodrin Antibody TA336859 - Mixed neuron-glial cultures stained with rabbit antibody to Alpha Fodrin (TA336859, green) and mouse polyclonal antibody to Nestin (red). The Alpha Fodrin antibody stains numerous

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 1:5000-1:10000, IF: 1:500-1:1000
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant construct containing the 7th, 8th and 9th spectrin repeats of human Alpha Fodrin (amino acids 676-1043). [UniProt# Q13813]
• Formulation: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Whole antisera
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 240 kDa
• Gene Name: spectrin alpha, non-erythrocytic 1
• Database Link:
  NP_001123910
  Entrez Gene 20740 MouseEntrez Gene 6709 Human
  UniProt ID Q13813
• Background: Alpha Fodrin (SPTAN1; also called non-erythroid alpha spectrin) belongs to spectrin family of proteins which are widely distributed filamentous proteins implicated in generating a supporting/organizing scaffold for cell membranes, and SPTAN1 protein is involved in processes such as secretion, interactions with calmodulin in Ca2+ dependent manner and Ca2+ dependent cytoskeletal movements at the membranes. SPTAN1 interacts with several proteins including ACP1, CALM, EMD, TRPC4 etc. and forms a component of a complex that contains several other proteins such as ACTN4, CASK, IQGAP1, MAGI2, NPHS1 and SPTBN1. SPTAN1 cleavage accompanies apoptosis, induced by activation via CD3/TCR complex, Fas/CD95 ligation and other Fas-expressing cells or through cellular exposure to staurosporine, dexamethasone, synthetic ceramide etc. Major products of SPTAN1 cleavage are N-terminal/C-terminal fragments, and phosphorylation of Tyr-1176 has been shown to decrease its sensitivity to cleavage by calpain in vitro. Defects in SPTAN1 have been linked to EIEE5 (epileptic encephalopathy early infantile type 5).
• Synonyms: EIEE5; NEAS; SPTA2
• Note: This Alpha Fodrin antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band can be seen at approximately 240 kDa.

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Tight junction