TRPM8 Rabbit Polyclonal Antibody

CAT#: TA336867

Rabbit Polyclonal TRPM8 Antibody

Product Images

Immunohistochemistry: TRPM8 Antibody TA336867 - Analysis of TRPM8 in mouse prostate using DAB with hematoxylin counterstain.

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  Immunocytochemistry/Immunofluorescence: TRPM8 Antibody TA336867 - TRPM8 antibody was tested in U2OS cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).

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  Western Blot: TRPM8 Antibody TA336867 - Analysis of TRPM8 in PC12 cell lysate.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Knockdown Validated, Immunocytochemistry/ Immunofluorescence: 1:100, Western Blot: 1:1000, Immunohistochemistry-Paraffin: 1:300, Immunohistochemistry: 1:200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A synthetic peptide made to an internal portion of the human TRPM8 protein (between residues 250-300) [UniProt Q7Z2W7]
• Formulation: PBS with 30% Glycerol, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: transient receptor potential cation channel subfamily M member 8
• Database Link:
  NP_076985
  Entrez Gene 171382 MouseEntrez Gene 171384 RatEntrez Gene 79054 Human
  UniProt ID Q7Z2W7
• Background: TRPM8 (TRPP8, LTRPC6) is a transient receptor cation channel that has been shown to be a major cold receptor in sensory neurons. TRPM8 has been theorized to be activated by cold temperatures, voltage, calcium store depletion, some lipids, and also the compounds that produce cooling sensations such as menthol or icilin. TRPM8 is aberrantly over-expressed in pancreatic cancer along with colon, lung, breast and skin cancers, and is proposed to be required for cellular proliferation.
• Synonyms: LTRPC6; TRPP8
• Note: This TRPM8 antibody is useful for Western Blot where a band is seen ~127 kDa, Immunocytochemistry/Immunofluorescence, and IHC-paraffin embedded sections. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Reference Data

• Protein Families: Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane